狂犬病固定毒Vero细胞适应株的建立  

The Adaptation of Rabies Virus, Strains in Vero Cell

作　　者：曾蓉芳[1] 陈阿根 张瑛 黄海武 

机构地区：[1]卫生部上海生物制品研究所病毒研究室,上海200052

出　　处：《病毒学杂志》1990年第3期285-290,共6页

摘　　要：本文报道狂犬病毒Vero细胞适应株建株经过以及对适应株的抗原和免疫原性分析的情况,并推荐“RFD”和“Hs_3”适应株作为狂犬疫苗微载体培养系统的候选毒株。Rabies is one of the serious infective diseases in China. More than 5 million people per year are wounded by animals. The yield of hamster kidney rabies vaccine produced in China every year is not enough to meet the practical need. So we attempt to develop a Veto cell rabies vaccine with microcarrier system for large scale production. In this paper, the 'aG' virus strain, a Chinese rabies fixed virus used for preparing primary hamster kidney cell vaccine in China since 1980, was adapted in Vero cell. 2 adapted strains 'RFD' and 'HS_3' grew well in Vero cell and the virus titre of the supernatants was about 10^(-6·5)/ml(LD_(50)). The antigenicity and immunogenicity of 'RFD' and 'HS_3' did not show significant variation from their parent virus 'aG' strain and have good cross-reaction with 'CVS' fixed virus, Both of them could induce the laboratory animal such as mice, rabbits and dogs to develop neutralizing antibodies. It is suggested that these adapted strains could be used for promising candidates of virus seed for Vero cell rabies vaccine producion.

关 键 词：狂犬固定毒 VERO细胞 适应传代 

分 类 号：R373.9[医药卫生—病原生物学]