大肠杆菌acrA基因的克隆、表达、抗体制备及不同耐药水平大肠杆菌AcrA表达水平的检测  

Cloning and expression of acrA gene and preparation antibodies and inspect the level of AcrA expression in different drug resistance level in Escherichia coli

作　　者：张海旺[1] 张丽云[1] 刘旭东 邓旭明[3] 胡仲明[4] 曾凡勤[3] 

机构地区：[1]河北北方学院,河北张家口075131 [2]河北省兽医药品监察所,河北石家庄040051 [3]吉林大学畜牧兽医学院,吉林长春130062 [4]军事医学科学院军事兽医研究所,吉林长春130062

出　　处：《中国预防兽医学报》2006年第3期279-284,共6页Chinese Journal of Preventive Veterinary Medicine

基　　金：国家自然科学基金资助课题(课题编号30270999)

摘　　要：检测不同耐药水平大肠杆菌AcrA表达水平,探讨耐药水平与AcrA蛋白表达水平之间的关系。对acrA基因进行克隆、表达、制备抗AcrA抗体,Westernblot方法比较同耐药水平大肠杆菌AcrA表达水平。结果表明,多重耐药株SEMR的AcrA表达明显高于单药耐药株SEICI和SEICH以及质控株ATCC25922。因此SEMR多重耐药性的产生与AcrA的高效表达直接有关。To inspect the level of AcrA expression in different drug resistance level in Escherichia coil,and discuss the relationship of resistance level with the expression level of AcrA protein. The acrA gene was cloned, AcrA protein was expression, and the anti- AcrA protein antibodies was prepared, at the same time , the expression level of AcrA protein in different drug resistance level in Escherichia coli was compared by Westem blot. The results showed that the expression level of AcrA protein in muti-drug resistance strain SEMR was higher than single-drug resistance strain SEICI and SEICH, control strain ATCC25922. There were relativity in the muti-drug resistance of SEMR with the higher expression level of AcrA.

关 键 词：大肠杆菌 多重耐药 AcrA 基因表达 

分 类 号：S852.612[农业科学—基础兽医学]