细粒棘球绦虫Eg95抗原编码基因在BCG中的表达效率研究  

Study on expression efficiency of the Echinococcus granulosus 95 antigen encoding gene in BCG

作　　者：朱佑明[1] 李文桂[1] 

机构地区：[1]重庆医科大学附属第一医院传染病寄生虫病研究所,重庆400016

出　　处：《中国病原生物学杂志》2006年第2期95-97,共3页Journal of Pathogen Biology

基　　金：国家自然科学基金(No.30200239);重庆市高等学校优秀中青年骨干教师资助项目(No.G0422)。

摘　　要：目的探讨细粒棘球绦虫Eg95抗原在BCG中的表达,为囊型包虫病疫苗的开发利用提供依据。方法用热诱导方法诱导rBCG-Eg95疫苗,用SDS-PAGE和Westernblot鉴定所表达的蛋白。结果表达重组蛋白为可溶性蛋白,分子质量单位为16.5ku。Westernblot示重组蛋白具有抗原性,用薄层扫描Bio-RadQuantityone分析系统分析,表达效率为占BCG菌体总蛋白的12%。结论细粒棘球绦虫Eg95抗原在BCG中成功表达,为BCG发展为多价疫苗载体奠定了基础。Objective To study the expression of the Echinococcus granulosus 95 （Eg95） antigen encoding gene in BCG. Methods Recombinant BCG-Eg95 vaccine was induced daily at 45℃ for 45 rain at the last there days, The product from BCG was analyzed by SDS-PAGE and Western blot, Results The expressed recombinant Eg95 was soluble and could be detected by SDS-PAGE about the molecular weight of 16.5 ku, and possessed antigenicity in Western blot . The expression efficiency of Eg95 was about 8 of the total bacterial protein in BCG, Conclusion The E. granulosus 95 antigen gene in BCG is successfully expressed. The study could provide basis to develop the BCG multivalent vaccine vectors.

关 键 词：细粒棘球绦虫 95抗原 BCG 大肠埃希菌 表达 

分 类 号：R383.33[医药卫生—医学寄生虫学]