脉冲场凝胶电泳用于霍乱弧菌分子流行病学研究  

Study on molecular epidemiology of Vibrio cholerae by pulse field gel electrophoresis

作　　者：詹銮峰[1] 严延生[1] 郭维植[1] 王晓萍[1] 陈亮[1] 陈亢川[1] 

机构地区：[1]福建省疾病预防控制中心,福州350001

出　　处：《中国卫生检验杂志》2006年第5期524-526,共3页Chinese Journal of Health Laboratory Technology

摘　　要：目的:从分子生物学基础上了解同一次流行、不同来源的霍乱弧菌之间的遗传相关性,为有效控制传染源提供依据。方法:采用PCR技术对2005年分离的70株霍乱弧菌检测CT基因后,进行PFGE分型研究。结果:分离自患者O1、O139群霍乱弧菌均有CT基因,而分离自外环境1株O1群稻叶菌株及1株O139菌株CT基因为阴性。所试菌株PFGE分型共呈3大亚类8种带型。结论:霍乱流行期间分离于患者的霍乱弧菌PFGE图谱基本一致,与外环境分离的非流行株在分化关系上较远。Objective：To understand the genetic correlation of Vibrio cholerae came from different source during the same cholera outbreak incident on the basis of molecular biology, in order to provide a basis for infectious source control. Methods：70 strains of Vibrio cholerae which were isolated in 2005 were detected cholera toxin（CT） gene by PCR, and the genotypes of strains were classified by pulse field gel electrophoresis（PFGE）. Results：O1 and O139 Vibrio cholerae strains isolated from patients were all CT positive, and 1 O1 EI Tor and 1 O139 strains isolated from the environment were CT negative. Three different subtypes and eight stip types were found among strains detected. Conclusion：The PFGE map of Vibrio cholerae strains isolated from patients during cholera epidemic were basically consistent, but differed greatly from non - epidemigenic strains isolated from the environment.

关 键 词：霍乱弧菌 脉冲场凝胶电泳 分型 

分 类 号：R378.3[医药卫生—病原生物学]