N型乙酰胆碱受体α10亚基在大鼠内耳耳蜗及前庭中的表达  

Expression of α10 Acetylcholine Receptor Subunit mRNA in the Rat Cochlea and Vestibular

作　　者：姚琦 孔维佳[1] 程华茂 吴婷婷 夏交 朱云 

机构地区：[1]华中科技大学同济医学院附属协和医院耳鼻咽喉科,武汉430022

出　　处：《听力学及言语疾病杂志》2006年第3期195-197,210,T0002,共5页Journal of Audiology and Speech Pathology

基　　金：国家杰出青年科学基金(39925035);卫生部临床学科重点项目资助(20012332);国家自然科学基金(30070809);高等学校博士学科点专项科研基金(20020487062)资助项目

摘　　要：目的研究N型乙酰胆碱受体α10亚基在大鼠前庭终器、Scarpa’s神经节、基底膜、螺旋神经节中的表达。方法运用RT-PCR技术分别检测大鼠前庭终器、Scarpa’s神经节、基底膜、螺旋神经节编码N型乙酰胆碱受体α10亚基的基因表达。以大鼠脑组织RNA为阳性对照。结果N型乙酰胆碱受体α10亚基在大鼠前庭终器、基底膜上有表达,在Scarpa’s神经节、螺旋神经节中无表达。结论在前庭终器和基底膜上扩增出标志N型乙酰胆碱受体α10亚基基因表达的PCR产物,为乙酰胆碱是内耳重要的神经递质之一提供了支持,并为N型乙酰胆碱受体在内耳中的传入-传出调节中的作用提供了依据。Objective To investigate the expression of the α10 acetyleholine receptor subunit mRNA in the rat inner ear vestibular end - organ, Scarpa＇ s ganglion , cochlea basement membrane and spiral ganglion. Methods Using a combination of reverse transcription followed by polymerase chain reaction（RT- PCR）, expression of the α10 aeetyleholine receptor subunit mRNA in the rat inner ear vestibular endorgan, Scarpa＇ s ganglion , cochlea basement membrane and spiral ganglion was examined. The brain RNA was used as positive control. Results α10 aeetylcholine receptor subunit mRNA was expressed in the rat vestibular end - organ and cochlea basement membrane, but was not expressed in the Scarpa＇ s ganglion and spiral ganglion. Conclusion The result indicates that acetylcholine is one of the important neurotransmitters in the inner ear, and plays a role in the neurochemical transmission subserving the efferent- afferent interaction in the inner ear.

关 键 词：N型乙酰胆碱受体α10 耳蜗 前庭 逆转录-聚合酶链反应技术 大鼠 

分 类 号：R339.16[医药卫生—人体生理学]