基因芯片技术分析20 cGy γ射线照射人淋巴母细胞中差异表达的基因谱  

SCREENING OF RADIATION-INDUCED GENES IN HUMAN LYMPHOBLASTOID CELLS IRRADIATED WITH 20 cGy OF γ-RAY BY GENE CHIP

作　　者：王会平[1] 龙贤辉[1] 徐勤枝[1] 白贝[1] 隋建丽[1] 周平坤[1] 

机构地区：[1]军事医学科学院放射与辐射医学研究所,北京100850

出　　处：《辐射防护》2006年第3期151-156,共6页Radiation Protection

基　　金：国家自然科学基金(No.30270423);国家高技术研究发展计划"863"(No.2004AA221160)资助项目

摘　　要：应用基因芯片技术对20 cGy 60Coγ射线照射的人淋巴母细胞和对照细胞中的mRNA表达水平进行对比杂交分析,探索差异表达基因。结果发现在所分析的14 112条目的基因中差异表达显著的基因(2倍以上差异)有83条,其中表达上调的有21条,表达下降的有62条,这些基因表达产物涉及到信号转导、细胞周期调节、免疫相关蛋白、细胞骨架与运动等功能基因。表明低剂量辐射对多种功能基因表达产生了影响,是调控细胞辐射反应最基本的分子基础。cDNA gene chip was used to detect the transcriptional profile of human lymphoblast cells irradiated with 20 cGy of ^60Co γ-ray. The microarray contains 14112 cDNA probing corresponding to 11412 human genes. The results showed that the transcription level of 83 genes changed, among which 21 genes were up-regulated. Most of them were associated with signal transduction, cell cycle regulation, cellular immunity, cytoskeleton and movement, etc. It indicated that low-dose irradiation can modulate the expression of a series of functional genes, which is the primary molecular basis of cellular responses to radiation.

关 键 词：基因芯片 低剂量辐射 基因表达 

分 类 号：R811.5[医药卫生—放射医学]