巢蛋白在人真皮成纤维细胞中的表达  

Nestin expression in fibroblasts of adult human dermis

作　　者：乔薇[1] 陈东明[1] 秦荣生[1] 赵霞[1] 

机构地区：[1]北京大学第三医院成形外科,北京100083

出　　处：《中国微创外科杂志》2006年第5期389-391,共3页Chinese Journal of Minimally Invasive Surgery

摘　　要：目的探讨巢蛋白(nestin)在人皮肤真皮组织成纤维细胞(adu lt hum an derm is fibrob lasts,HDF)中的表达。方法采用体外培养、免疫组织/细胞化学技术检测巢蛋白在6例成人皮肤真皮组织及体外培养3、5、7、10、12代龄皮肤真皮成纤维细胞中的表达,计数分析人皮肤真皮组织成纤维细胞中巢蛋白+成纤维细胞数量,权重法分析体外培养成纤维细胞各代间巢蛋白的表达差异。结果人皮肤真皮组织中,巢蛋白+成纤维细胞表达数量占成纤维细胞总数(9.5±3.0)%,其数量为(103.3±67.4)个/mm2。与人皮肤真皮组织巢蛋白+成纤维细胞的表达数量相比,体外培养的真皮成纤维细胞中巢蛋白+成纤维细胞数量明显升高,并出现代龄之间的差异(P<0.05),其中第7、10代细胞表达量高于第5、12代细胞,第3代细胞最少(P<0.05)。结论在人皮肤真皮组织成纤维细胞中存在着巢蛋白+成纤维细胞。体外培养条件下,人皮肤真皮巢蛋白+成纤维细胞数量增加,提示体外培养作为刺激因子可能使成纤维细胞反分化为其前体细胞,作为干细胞标志物的巢蛋白有可能成为用于成纤维前体细胞鉴定的标志物之一。Objective To investigate the expression of nestin in fibroblasts of adult human dermis. Methods A total of 6 samples of normal human skin were collected. Immunohistochemistry and immunocytochemistry staining were used to detect the nestin expression in adult human dermis and in cultured fibroblasts of different passages （3rd, 5th, 7th, lOth, and 12th passages, respectively） in vitro. Nestin positive cells were counted to evaluate the expression quantity and intensity. Results There were 103.3 ± 67.4 fibroblasts per square millimeter （ mm^2 ） of adult human dermis, and the nestin positive fibroblasts accounted for （9.5 ± 3.0） % of total amount. The amount of nestin positive fibroblasts in vitro was obviously higher than that in vivo. Significant difference was observed in the amount of nestin expression among different passages （P 〈 0.05 ）. The highest expression quantity was observed in the 7th and 10th passages, moderate in the 5th and 12th, and the lowest in 3th （P 〈 0.05 ）. Conclusions There are nestin positive fibroblasts in normal adult human dermis. The amount of nestin positive fibroblasts in vitro is obviously higher than that in vivo. Since nestin expression is successfully induced in cultured fibroblasts, it is suggested that fibroblasts be promoted to dedifferentiate into their precursors by cell culture which acts as a stimulative factor. A biomarker of stem cells, nestin is expected to act as a biomarker in fibroblasts precursor for identification.

关 键 词：巢蛋白 真皮 成纤维细胞 前体细胞 

分 类 号：R329[医药卫生—人体解剖和组织胚胎学]