巴氏杆菌可溶性抗原的提取及蛋白浓度测定  

Extract of Soluble Antigens and Protein Contents Measurement in Pasteurell

作　　者：毛东风[1] 刘翊中[1] 佟辉[1] 孙会芳[1] 冯若飞[1] 王建[1] 

机构地区：[1]西北民族大学生命科学与工程学院,甘肃兰州730030

出　　处：《西北民族大学学报（自然科学版）》2006年第1期64-66,共3页Journal of Northwest Minzu University(Natural Science)

摘　　要：改良EDTA法提取巴氏杆菌内可溶性抗原成分,经透析浓缩后保存,并取用考马斯亮蓝法测定其浓度.结果得回归方程为y=0.5589x-0.0262,r=0.9990.由吸光度推出样品蛋白浓度为1.643mg/mL,而蛋白检测仪为1.412mg/mL.The soluble antigens of Pasteurella were extracted by improved EDTA method and were preserved after having been dialysed and concentrated. The consistence of the soluble antigens were measured by Coomassie Brilliant Blue method. Then the tropic equation came out to be： y= 0. 5589x- 0. 0262, r= 0. 9990. So the protein consistency of tile sample was figured out to be 1. 643 mg/mL according to the absorbence, While the result come from Pritein Measureing Instrument was 1. 412 mg/mL.

关 键 词：EDTA 考马斯亮蓝 ELISA 

分 类 号：S852.6[农业科学—基础兽医学]