清肝化瘀方药、苦参素对大鼠肝癌形成过程中ERK蛋白的影响  

Effect of Liver-clearing and Stasis-removing Decoction and Matrine on ERK in Hepatocellular Carcinoma of Rats

作　　者：殷飞[1] 姚树坤[1] 吴新满[1] 

机构地区：[1]河北医科大学第四医院消化科,河北石家庄050011

出　　处：《中国中医基础医学杂志》2006年第4期270-275,共6页JOURNAL OF BASIC CHINESE MEDICINE

基　　金：河北省自然科学基金资助(301394)

摘　　要：目的:观察清肝化瘀方药阻断大鼠肝癌前病变和癌变的作用及其对信号蛋白ERK的影响。方法:以二乙基亚硝胺长期灌胃诱发大鼠肝癌,以苦参素做对照,进行癌前病变及癌变期HE染色、γ-谷氨酰转肽酶(GGT)组化染色,ERK免疫组化。结果:清肝化瘀方药和苦参素癌前病变及癌变期病理改变较病理组明显减轻。癌前病变期病理组肝组织GGT酶组化染色单位切面病灶数、单位面积内病灶面积、病灶平均面积3项指标显著高于各药物预防组(P<0.05或P<0.01)。单位切面病灶数、单位面积内病灶面积清肝化瘀方药组低于苦参素组(P<0.05)。癌前病变期和癌变期清肝化瘀方药组ERK在肝细胞胞浆和胞核着色较病理组明显减轻,毛细胆管轻微着色,苦参素组ERK在肝细胞异型增生灶、癌巢周围及毛细胆管均着色,但较病理组轻。结论:清肝化瘀方药能阻断二乙基亚硝胺所致大鼠肝癌前病变和癌变的发生,效果优于苦参素。清肝化瘀方药能降低MAPKs通路中通路的活性,抑制细胞过度增殖。Objective： To investigate the repressive effect of（LCSRD） on genesis of HCC induced by diethylnitrosamine（DEN） and expression of MEK in precancerous and cancerous lesion of rats liver. Methods： Male rats were fed with DEN water solution 35mg/kg twice a week for 16 weeks and LCSRD 20 weeks, using Matrine as control. All rats were sacrificed at 12^th, 20^th experimental week respectively. Hematoxylin-eosin （HE） staining, Histochemistry of γ-glutamyl transpeptidase （GGT） positive foci, ERK expression by iummnohistochemistry （IHC） staining in liver tissue were observed to compare the effect of the drugs. Results： At 12^th and 20^th experimental week, pathologic changes in LCSRD group and Matrine group were more milder than those in pathologic group significantly. The three indexes about numbers and areas of GGT positive foci were significantly higher in pathologic group than those in LCSRD group and Matrine group （ P 〈 0.05 or P 〈 0.01 ） .The indexes about number/cm^2 , mm^2/cm^2 of GGT positive foci in GZPS group were lower than those in Matrine group（ P 〈 0.05）. The expression of ERK in pathologic group was in hepatocyte dysplasia focies of pseudolobules and bile capillaries, and that in Matrine group was much slighter. There was little expression of ERK in LCSRD group. Conclusions ： LCSRD is of obvious repressive effect on the genesis of HCC in rat. The prevention effect of GanZheng solution on HCC was better than that of matrine. LCSRD could repress the expression of ERK in HCC, which was one of the major mechanisms of anticareinogenesis.

关 键 词：清肝化瘀方药 苦参素 肝癌形成 ERK 

分 类 号：R273.57[医药卫生—中西医结合] R285.5[医药卫生—中医肿瘤科]