电针足阳明经穴对大鼠胃黏膜损伤修复机制的研究  

Mechanism of Study on Gastric Mucosal Lesion Restoration in Rats Treated by Electroacupuncture

作　　者：严洁[1] 黎喜平[1] 黄艾[1] 易受乡[1] 常小荣[1] 林亚平[1] 胡蓉[1] 

机构地区：[1]湖南中医学院,湖南长沙410007

出　　处：《中国中医药信息杂志》2006年第5期20-22,共3页Chinese Journal of Information on Traditional Chinese Medicine

基　　金：国家自然科学基金重点项目(90209023);国家973计划中医基础研究资助(2005-CB523305)

摘　　要：目的比较电针足阳明、足少阳经穴对实验性胃溃疡大鼠胃黏膜损伤修复作用的异同,探讨足阳明经与胃相关的特异性及电针足阳明经穴对胃黏膜损伤细胞修复作用的可能机制。方法将40只大鼠随机分为空白组、模型组、胃经组、胆经组。造模采用水浸束缚法。实验结束后取血清及胃黏膜组织,采用放射免疫分析法测定表皮生长因子(EGF)、转化生长因子α(TGF-α)含量,采用逆转录-聚合酶链反应法(RT-PCR法)分析胃黏膜EGFRmRNA表达。结果电针足阳明经穴均能使胃黏膜损伤指数显著降低,能显著升高胃黏膜EGF、TGF-α含量;电针上调EGFRmRNA在胃黏膜的表达水平,其中以足阳明经穴组作用最强,与足少阳经组比较差异显著。结论电针足阳明经穴对大鼠胃黏膜损伤的细胞修复作用是通过促进EGF、TGF-α等相关因子的合成、分泌与释放实现的;增强EGFRmRNA在胃黏膜局部的表达,可能是电针足阳明经穴对大鼠胃黏膜损伤修复作用的另一机制;足阳明经与胃具有相对的特异性。Objective Through comparing the similarities and differences between respalring effect on rat＇s gastric mucosal lesion by electroacupure （EA） at Meridian of Foot Yangming and Meridian of Foot Shaoyang, to explore the specificity of the correlation between Meridian of Foot and Stomach and the mechanism of the healing effect by EA at meridian of Foot Yangming for curing the gastric lesion. Methods Forty rats were randomly divided into 4 groups： blank group, model group, stomach tunnel group, gallbladder tunnel group. All rats （except normal group） were made model by water restraint stress （WRS）. After corresponding experimental process, epidermal growth factor （EGF） and transforming growth factor alpha （TGF-α） of plasma, gastric mucous tissue concentration was detected with radioimmunoassay, and the expression of EGFR mRNA of above tissue was detected by the revekse transcriptase-polymease chain reaction （RT-PCR） method.Resnlts The gastric mucosal lesion index of Foot Yangming group decreased significantly in comparison with that of other groups, while EGF, TGF-α of gastric mucous tissue concentration level of Foot Yangming group was obviously higher than that of model group and gallbladder tunnel group. EA oould up-regulate the expression gastric mucosal EGFRmRNA, and there was a more significant difference in the group by EA at Meridian of Foot Yangming than that in the model group and gallbladder tunnel group. Conclusion The curative effect of EA at points of Meridian of Foot Yangrning on the gastric mucosa may be realized by the synthesis, secretion and release of the related EGF family factor, such as EGF, TGF-α and so on. Such special regulation of electroacupuncture on gastric mucosal tissue was related to the gene expression of EGFRmRNA, and that may be further mechanism of the curative effect of EA at points of Meridean of Foot-Yangming on gastric mucosal lesion. There existed corresponding correlation between Meridian of Foot Yangming and Stomach.

关 键 词：电针 胃黏膜损伤 表皮生长因子 转化生长因子Α 应激 表皮生长因子受体 基因表达 

分 类 号：R285.5[医药卫生—中药学]