清热化瘀方对内毒素血症家兔血管内皮细胞分泌功能的影响  

Influence of Qingre Huayu Recipe(QHR) on Secretive Function of Vaso-endothelial Cells in Rabbits with Endotoxemia

作　　者：杨爱东[1] 沈庆法[1] 郭永洁[1] 陈洪[1] 

机构地区：[1]上海中医药大学温病教研室,上海201203

出　　处：《中国中医药信息杂志》2006年第5期25-27,共3页Chinese Journal of Information on Traditional Chinese Medicine

基　　金：上海市教育委员会自然基金资助项目(97CJ03)

摘　　要：目的探讨清热化瘀方(主药:生石膏、知母、赤芍)对家兔内毒素血症血管内皮细胞分泌功能的影响。方法实验采用内毒素血症(大肠杆菌内毒素从家兔耳缘静脉注射,造成内毒素血症)家兔模型。观察模型组及清热化瘀方组病理学及血管内皮细胞分泌功能的改变。结果模型组光镜下肺脏有微血栓存在,且模型组家兔组织纤溶酶原激活物(t-PA)/组织纤溶酶原激活物抑制剂(PAI)、一氧化氮(NO)/内皮素(ET)、血栓烷B2(TXB2)/6-酮-前列腺素F1α(PGF1α)平衡失调。与模型组相比,清热化瘀方组脏器病变轻微,且未见微血栓形成,t-PA/PAI、NO/ET、TXB2/6-酮-PGF1α平衡失调改善(P<0.01)。结论清热化瘀方能阻断微血栓的形成,推测其机理可能与调节内皮细胞释放的血管活性物质如ET/NO、t-PA/PAI、TXB2/6-酮-PGF1α之间的平衡及拮抗微血栓形成的病理损害有关。Objective To explore the mechanism of QHR （main ingredients： Gypsum, Anemarrhema Asphodeloides Bge, Paeonia Lactiflora Pall） on secretive function of vaso-endotheliai cells in rabbits with of endotoxemia. Methods Endotoxemia model was induced by colibacillary endotoxin via auricular intravenous injection in rabbits. The changes on pathomorphology and secretive function of vaso-endothelial cells in the model and QHR group were observed, Results It showed the existence of microthrombus in the lung via microscope in the model. The balance destruction of t-PA/PAI, NO/ET, TXB2/6-K-PGF1α in the model （P〈0.01）. The microthrombus in the lung of QHR group was lessand the balance destruction of t-PA/PAI, NO/ET, TXB2/6-K-PGF1α in the QHR was impoved （P〈0.01）. Conclusion The blood stasis was stopped by QHR. The mechanism may be the regulation of the balance of t-PA/PAI,NO/ET, TXB2/6-K-PGF1α and perhaps repress microthrombus.

关 键 词：清热化瘀方 内毒素血症 组织纤溶酶原激活物 组织纤溶酶原激活物抑制剂 一氧化氮 内皮素 血栓素B2 6-酮-前列腺素F1Α 

分 类 号：R285.5[医药卫生—中药学]