The Synthesis and Characterization of β-cyclodextrin Derivated Pancreatin Used as Chiral Selector in Capillary Electrochromatography  

作　　者：戴荣继 

机构地区：[1]School of Life Science and Technology, Beijing Institute of Technology, Beijing 100081, China

出　　处：《Journal of Wuhan University of Technology(Materials Science)》2005年第B12期27-28,共2页武汉理工大学学报（材料科学英文版）

基　　金：FundedbytheNationalNaturalScienceFoundationofChina(20275004)andtheBasicResearchFoundationofBeijingInstituteofTechnology

摘　　要：The preparation of capillary electrochromatography column for chiral separation with β-cyclodextrin derivatized Pancreatin was imvestigated, which had three steps, synthesis of β-cyclodextrin derivated pancreatin, the modification of the capillary internal surface and the covalent binding of β-cyclodextrin derivated pancreatin on the capillary internal wall. β-cyclodextrin derivated pancreatin was synthesized using β-cyclodextrin and protein in the presence of ethylene glycol diglycidyl ether （EGDE） in boric acid buffer at pH = 8.7. Amino group was coated on the internal surface of the silica capillary by sot-gel technology using triethoxylmethylsiloxane and （ 3-aminopropyl ） trimethoxysiloxane. Covalent binding of β-cyclodextrin derivated pancreatin was performed by glataraldehyde. Chlorpheniramine, phenylalanine and troopine were separated baseline by β-cyclodetrin derivated pancreatin in capillary electrochromatography.The preparation of capillary electrochromatography column for chiral separation with β-cyclodextrin derivatized Pancreatin was imvestigated, which had three steps, synthesis of β-cyclodextrin derivated pancreatin, the modification of the capillary internal surface and the covalent binding of β-cyclodextrin derivated pancreatin on the capillary internal wall. β-cyclodextrin derivated pancreatin was synthesized using β-cyclodextrin and protein in the presence of ethylene glycol diglycidyl ether （EGDE） in boric acid buffer at pH = 8.7. Amino group was coated on the internal surface of the silica capillary by sot-gel technology using triethoxylmethylsiloxane and （ 3-aminopropyl ） trimethoxysiloxane. Covalent binding of β-cyclodextrin derivated pancreatin was performed by glataraldehyde. Chlorpheniramine, phenylalanine and troopine were separated baseline by β-cyclodetrin derivated pancreatin in capillary electrochromatography.

关 键 词：capillary electrochromatography PANCREATIN chiral separation Β-CYCLODEXTRIN 

分 类 号：Q578[生物学—生物化学]