长骨液对骨延长区BMP影响的实验研究  

Experimental Study of the Effect of ＂Bone Growth Fluid＂ in the Osteogensis of Bone Lengthening Area

作　　者：邹培[1] 李竣辉[1] 周中英[1] 李主一[1] 林月秋[1] 阮默[1] 

机构地区：[1]中国成都军区昆明总医院全军骨科中心,昆明650032

出　　处：《世界中医骨科杂志》2005年第2期144-145,51,共3页World Journal of Traditional Chinese Orthoprdics

摘　　要：目的：为了观察“长骨液”对骨延长区成骨作用的影响。方法：实验家兔54只造成左胫骨中段骨延长模型。并随机分为对照组和实验组。试验组于术后第二天开始喂含中药长骨液的颗粒饲料。对照组则喂普通饲料。定期取材，按常规做成光镜切片，光镜下观察。同时用抗骨形成蛋白（BMP）单抗作免疫组化染色。结果：实验组新骨形成及成熟较对照组提前。结论：长骨液能够明显地促进骨延长区BMP的合成与分泌，加速新骨的形成与成熟。Objective：In order to study the of chines traditional medicine ＂bone-growth fluid＂ on bone formation in bone lengthening. Methods： Bone lengthening models were produced in the middle part of the left bibia of 54 rabbits, which were divided into control group and experiment group.The rabbits in the experiment group were given feedstuff containing Zhanggu Fluid at the second day after operation and those in control group were fed with normal feedstuff.In the scheduled time the interesting bone tissue.Slices were made and observed under optical microscopy. Bone morphogentic protein （BMP） was detected immunohistochdmically using monoclonal antibody against BMP. Results：The results showed-that new bone formation and bone remodeling in the experimental group appeared earlier than that in the control group. Conclusion：The chinese medicin ＂Bone Growth-Fluid＂ can obviously plays a role in accelerating the promoting osteogenesis and bone remodeling in bone lengthening.

关 键 词：长骨液 骨延长 骨形态发生蛋白 实验研究 

分 类 号：R274[医药卫生—中医骨伤科学] R285.5[医药卫生—中医学]