蝉拟青霉总多糖对免疫抑制大鼠组织器官免疫功能调节的实验研究  

Paecilomyces cicadidae total polysaccharides regulates immune function in immunosuppressed rats

作　　者：金丽琴[1] 吕建新[2] 杨介钻[2] 李东[2] 李安乐[3] 

机构地区：[1]温州医学院临床医学部生物化学教研室 [2]温州医学院细胞与分子医学研究所 [3]温州医学院实验动物中心,浙江温州325035

出　　处：《中国病理生理杂志》2006年第5期881-884,共4页Chinese Journal of Pathophysiology

基　　金：浙江省教育厅科研基金资助项目(No.20020471);温州市"551"人才基金资助项目

摘　　要：目的:观察蝉拟青霉总多糖对免疫抑制大鼠组织器官免疫功能调节和自由基代谢的影响,探讨其对免疫抑制大鼠组织器官的免疫调节和抗衰老作用。方法:以速率法在全自动生化分析仪上测定乳酸脱氢酶(LDH)活力;以鸟氨酸的生成判断精氨酸酶(arginase)的活力;以硫代巴比妥酸-醋酸法测定脂质过氧化物(LPO)含量;以Ell-man试剂测定还原型谷胱甘肽(GSH)水平。结果:蝉拟青霉总多糖使环磷酰胺所致免疫抑制大鼠组织细胞内LDH(肾、胸腺)、arginase(肝、脾、胸腺)活力升高,具有显著差异(均P<0.01);使LPO(肾、脾、胸腺)的含量下降(P<0.05或P<0.01);使环磷酰胺所致的肝、脾内低下的还原型谷胱甘肽(GSH)升高(均P<0.01)。结论:蝉拟青霉总多糖能增强免疫抑制大鼠组织器官的免疫功能和加速自由基的代谢。AIM： To study the effects of paecilomyces cicadidae total polysaccharides on the immune function and free radical scavenging of tissues in immunosuppressed rats. METHODS： The activities of lactate dehydrngenase （LDH） and arginase （Arg） in liver, kidney, spleen, thymus of immunosuppressed rats were detected by automatic biochemistry analyzer. The content of lipid peroxide （LPO） and the reduced glutathione （GSH） level in hver, kidney, spleen, thymus of immunosuppressed rats were observed. RESULTS： The activity of the LDH, Arg and GSH level were significantly increased, and the LPO content were significantly declined in liver, kidney, spleen, thymus of immunosuppressed rats induced by cyclophosphamide. CONCLUSION： Paecilomyces cicadidae total pelysaccharides promote immune function and the ability of free radical scavenging of tissues in immunosuppressed rats.

关 键 词：拟青霉 多糖类 免疫抑制 过氧化脂质类 谷胱甘肽 

分 类 号：R967[医药卫生—药理学]