慢性吸烟大鼠肺组织FIZZ1/REL Mα表达的变化  

Chronic cigarette smoking induces alteration of FIZZ1/RELMα expression in rat lung

作　　者：叶红[1] 马万里[2] 张少华[1] 徐清[1] 付世欧[1] 刘声远[1] 王迪浔[1] 

机构地区：[1]华中科技大学同济医学院病理生理系卫生部呼吸系疾病重点实验室 [2]华中科技大学同济医学院附属协和医院呼吸内科,湖北武汉430030

出　　处：《中国病理生理杂志》2006年第5期938-942,共5页Chinese Journal of Pathophysiology

摘　　要：目的:研究吸烟大鼠肺组织中FIZZ1/RELMα表达的变化及其与气道炎症和气道高反应性的关系。方法:复制大鼠的慢性吸烟模型,采用气道反应性测定、HE染色、免疫组织化学染色、原位杂交等方法,研究慢性吸烟大鼠肺组织中FIZZ1/RELMα表达的变化。结果:在正常对照组,RELMα蛋白呈散在分布,吸烟组支气管平滑肌、肺血管壁、肺泡上皮和肺间质的表达明显增强,吸烟组RELMαmRNA的表达在各种细胞均明显增强,其中以肺泡上皮、肺动脉内皮和支气管平滑肌的表达增强最为明显。结论:FIZZ1/RELMα作为肺部特异性表达的一种因子,吸烟使其表达增强,与吸烟引起的气道高反应性、肺血管反应性增强密切相关。AIM： To investigate the expression of FIZZ1/RELMα in lung tissue of chronic cigarette smoking rat, and to determine the relationship between airway inflammation and airway hyperresponsiveness. METHODS： Made rat model of chronic cigarette smoking was used. The expression of FIZZ1/RELMα in lung tissue was determined by immuno - histochemistry and in situ hybridization. RESULTS. In control rats, FIZZ1/RELMα protein and mRNA expressions were observed at low levels. In cigarette smokigg rats, FIZZ1/RELMα expression increased in all the cells especially in bronchial smooth muscle cells, vascular wall cells and alveolar epithelial cells. CONCLUSION： FIZZ1/RELMα is a secreted peptide specifically expressed in lung. Cigarette smoking induces its upregulation, which possibly contributes to cigarette smoking- induced airway hyperresponsiveness.

关 键 词：吸烟 气道 肺 基因 FIZZ1/RELMα 

分 类 号：R163[医药卫生—公共卫生与预防医学] R363