白藜芦醇苷对慢性常压低氧性肺动脉高压模型中磷脂酶A_2、一氧化氮和内皮素水平的影响  

Effects of polidatin on phospholipase A_2,nitric oxide and endothelin1 levels in serum and lung of hypoxic pulmonary hypertension rats

作　　者：陈莉延[1] 麦奇[2] 彭勃[1] 郭兰萍[1] 梁标[1] 

机构地区：[1]广东医学院呼吸疾病研究所 [2]广东医学院附属医院检验科,广东湛江524021

出　　处：《中国病理生理杂志》2006年第5期953-955,共3页Chinese Journal of Pathophysiology

基　　金：广东省科委自然科学基金资助项目(No.960761);广东医学院青年基金资助项目(No.200104/k01061)

摘　　要：目的:观察白藜芦醇苷(PD)对慢性常压低氧性肺动脉高压大鼠血浆及肺匀浆中磷脂酶A2(PLA2)、一氧化氮(NO)和内皮素1(ET-1)水平的影响,并探讨可能的机制。方法:29只健康SD大鼠随机分为正常对照组、单纯低氧组和低氧加PD组。右心导管法检测大鼠肺动脉平均压力(mPAP),观察右室/左室+室间隔重量比值(R/L+S)、血浆及肺匀浆中PLA2活性、NO和ET-1含量的变化。结果:低氧21 d后大鼠mPAP、R/L+S、血浆及肺匀浆中PLA2活性和ET-1含量显著高于对照组,NO含量显著低于对照组。PD预处理组上述变化可受抑制或减轻。结论:PD可有效防治慢性常压低氧性大鼠肺动脉压力的升高,其机理与抑制PLA2活性及ET-1释放,促进NO产生有关。AIM： To investigate the effects of polidatin on phespholipase A2 （PLA2）, nitric oxide （NO）, and endothelin - 1 （ ET- 1 ） levels in serum and lung homogenate of hypoxic pulmonary hypertension rats. METHODS： 29 healthy Spraugue - Dawhy rats were randomly divided into normal control group, chronic hypoxic group and hypoxic plus polidatin group. The model of chronic hypoxic pulmonary hypertension was made by method of intermittent isobaric hypoxia for 21 days. The mean pulmonary arterial pressure （mPAP） was measured by inserting a microcatheter into the pulmonary artery. The ratio of right ventricular wall and that of left ventricular wall and ventricular septum weight （R/L＋ S） was measured as well. RESULTS： After exposed to hypoxia for 21 days, the mPAP, R/L ＋ S, PLA2 and ET- 1 activities in blood and lung homogenate increased significantly. The NO concentration decreased significantly. Pretreatment with PD attenuated these changes mentioned above. CONCLUSION： Pretreatment with PD effectively prevents the formation of chronic hypoxic pulmonary hypertension. Its mechanism may be related to the inhibition of PLA2 and ET- 1 and promotion of NO production.

关 键 词：白藜芦醇苷 肺动脉高压 磷脂酶A 一氧化氮 内皮缩血管肽1 

分 类 号：R543.2[医药卫生—心血管疾病]