脯氨酸测定方法的改进  

Improvement of the Method for Measuring Proline Content

作　　者：职明星[1] 李秀菊[1] 

机构地区：[1]河南科技学院农业生物工程研究所,河南新乡453003

出　　处：《河南科技学院学报》2005年第4期10-12,共3页Journal of Henan Institute of Science and Technology(Natural Science Edition)

基　　金：河南省高校创新人才培养工程项目(2001513)

摘　　要：通过对酸性茚三酮法测定脯氨酸测定方法的研究,结果表明:25m g.mL-1的酸性茚酸酮溶液可以保存147d;当脯氨酸含量为100 ug时,充分萃取它与茚D酮反应的产物至少用7mL甲苯。The method for measuring praline content wilh Aeidic-ninhydrin was studied. The results showed that 25 mg·mL^-1 acidic ninhydfin could be kept in the fridge for 147 days. When the conceritration of pra line was 100 ug at least ,TmL toluene would be used to extract the reaction production of acidic-ninhydfin and praline.

关 键 词：脯氨酸 酸性茚三酮 测定方法 

分 类 号：O657.32[理学—分析化学]