VEGF、ET-1在供体活细胞诱导长期存活的移植物中的表达  

The expressions of VEGF and ET-1 in the long term survival heartgrafts induced by donor live cell injection

作　　者：孙岩[1] 韩德恩[1] 姜明山[1] 韩福友[1] 

机构地区：[1]哈尔滨医科大学附属第二医院小儿外科,150086

出　　处：《中华小儿外科杂志》2006年第5期259-261,共3页Chinese Journal of Pediatric Surgery

基　　金：黑龙江省自然基金资助项目(D200565)

摘　　要：目的探讨供体活细胞输注对移植物慢性排斥反应的影响及长期存活的移植物中VEGF、ET-1的表达。方法建立Wistar→SD大鼠心脏移植排斥反应模型,于术中经门静脉注射供体活细胞(脾细胞、骨髓细胞、肝细胞)诱导特异性免疫耐受,观察生存时间,对长期存活移植物进行病理形态学分析及半定量逆转录聚合酶链反应(RT-PCR)检测。结果同时注射两种或两种以上供体活细胞可使移植物长期存活(>90d)。长期存活的移植物血管病变明显减轻,仅有轻度炎症细胞浸润和间质纤维化,与CsA组及单一细胞注射组相比差异具有显著性意义(P<0.05)。联合注射组VEGF、ET-1表达明显减低。结论经门静脉注射两种或两种以上供体活细胞可减轻慢性排斥反应,VEGF、ET-1的表达与血管硬化及纤维化程度呈正相关。Objective To investigate the effects of donor live cells injection via portal vein on the chronic reiection of heartgrafts and the expressions of VEGF and ET-1 in the long term survival grafts. Methods SD recipients were treated with donor live cells injection via portal vein after receiving Wistar heartgraft to induce specific immunological tolerance. The grafts survival time was recorded. The morphological changes of long term survival grafts were analyzed. And the expressions of VEGF and ET-1 were assessed by reverse transcription-polymerase chain reaction. Results Injection of two or more kinds of donor cells could prolong the heartgraft survival （〉90 d）. The long-lived grafts showed less vasculopathy, fewer inflammatory cell infiltration, and smaller interstitial fibrosis compared to the CsA or mono-cell injection group （P〈0. 05）. The expressions of VEGF and ET-1 in combined injection groups were significantly lower than those in the mono-cell injection group. Conclusions Injection of two or more kinds of donor live cells may alleviate chronic rejection via decreasing of expressions of VEGF and ET-1 in the grafts.

关 键 词：门静脉 移植物排斥 

分 类 号：R726.5[医药卫生—儿科]