抗体偶联白蛋白声学微气泡的方法学研究  

Methodology of Antibody Conjugating Albumin Microbubbles Sonocated Contrast Agent

作　　者：刘娅妮[1] 邓又斌[1] 项光亚[2] 刘冰冰[1] 张奇[2] 龙娜[2] 

机构地区：[1]华中科技大学同济医学院附属同济医院超声影像科,武汉市430030 [2]华中科技大学同济医学院药学院

出　　处：《中国超声医学杂志》2006年第5期321-323,共3页Chinese Journal of Ultrasound in Medicine

摘　　要：目的制备抗体偶联的白蛋白声学微气泡造影剂,并探讨偶联反应的条件。方法采用N-succinimidyl3-(2-pyridyldithio)propionate,SPDP)法将人IgG抗体与白蛋白声学微气泡以不同的质量比进行共价偶联,玻片凝集法与免疫荧光染色法检验抗体与白蛋白声学微气泡造影剂的交联并确定最佳反应比例。结果SPDP法制备的免疫微气泡的玻片凝集反应以及免疫荧光染色均呈阳性,IgG抗体与白蛋白声学微气泡造影剂质量比为1∶5时免疫荧光反应强度最高。结论应用SPDP法能够将抗体有效地结合到白蛋白声学微气泡造影剂表面,为进一步靶向性声学微气泡造影剂的制备奠定了基础。Objective This study was performed to prepare albumin microbubbles bound with antibody by Nsuccinimidyl 3- （2-pyridyldithio） propionate （SPDP） conjugation technology and to explore the most proper reaction conditions. Methods The human lgG was coupled to albumin microbubbles on different quality ratios by SPDP. Slide agglutination and immunofluorescent assay were used to detect whether antibody bound with albumin microbubbles and to determine the best ratio. Results The prepared immunomicrobubbles were positive in slide agglutination and immunofluorescent assay. When the quality ratio was 1 ： 5, there was brightest reaction in immunofluorescent assay. Conclusions The antibody can be effectively attached to the surface of albumin microbubbles by SPDP conjugation technology, which may settle a foundation for further studies on preparation of targeted microbubbles.

关 键 词：抗体 微气泡 白蛋白 交联剂 

分 类 号：R91[医药卫生—药学]