肿瘤抗原冲击致敏的树突状细胞在骨髓移植小鼠中抗白血病的免疫反应  

Dendritic cells pulsed with whole tumor lysates provide protection against acute leukemia in syngeneic bone marrow-transplanted mice

作　　者：姜义荣[1] 刘春生[2] 陈学良[2] 马道新[2] 

机构地区：[1]广东省东莞市人民医院血液内科,广东东莞523018 [2]山东大学齐鲁医院,山东济南250012

出　　处：《中国实用内科杂志：临床前沿版》2006年第1期96-98,共3页

基　　金：国家自然科学基金资助项目(No.30070321)

摘　　要：目的 研究肿瘤抗原冲击致敏（pulsed with whole tumor lysates，TP）的树突状细胞（dendritic cells，DC）在骨髓移植（bone marrow transplantation，BMT）小鼠中诱导特异性的抗白血病免疫反应。方法 2002—06～2003—06在山东大学齐鲁医院使用mGM—CSF＋mIL-4培养诱导骨髓细胞分化，将L7212白血病细胞的冻融抗原冲击致敏DC，培养制备肿瘤抗原冲击致敏的树突状细胞（tumor lysates pulsed dendritic cells，TP—DC），将TP—DC重复免疫BMT后615小鼠，同时设立对照组，观察各组小鼠的生存期及存活率。结果 TP—DC能诱导产生针对L7212白血病细胞的特异性细胞毒性，与对照组相比差异显著（F=391，77，P〈0．01）。TP—DC重复免疫组小鼠的生存期及存活率明显高于对照组，差异显著（χ^2=18．14，P〈0．01）。结论 TP—DC重复免疫在BMT小鼠中产生明显的抗白血病免疫反应.Objective To investigate specific anti - leukemia immune response induced by tumor lysates pulsed dendritic cells （ TP - DC ） in syngeneic bone marrow - transplanted mice. Methods From June 2002 to June 2003, the bone marrow cells were induced by using granulocyte - macrophage colony stimulating factor （ mGM - CSF） and interleukin - 4 （mIL -4） . DC was pulsed with L7212 tumor lysates at the immature stage. TP - DC was harvested on 7th day. 615 mice immunized thrice with L7212 TP - DC starting at day 7 following BMT. Additional control groups were set up. The survival was observed. Results Immunization using TP - DC could induce L7212 cells - specific CTL responses that were statistically significant compared with control groups （ F = 391.77, P 〈0. 01 ） . The survival of leukemia - bearing mouse injected with TP- DC was significantly prolonged compared with control mouse （χ^2 = 18. 14, P 〈 0. 01 ）. Conclusion TP - DC vaccines appear to promote immune priming events in a setting of syngeneic BMT - induced lymphopenia that can result in anti -leukemia effects in vivo.

关 键 词：树突状细胞 骨髓移植 白血病 

分 类 号：R5[医药卫生—内科学]