不同类型造血干细胞移植后巨细胞病毒感染的监测  

Monitoring the incidence of CMV infection after different stem cell transplantation.

作　　者：吴小津[1] 吴德沛[1] 孙爱宁[1] 常惠荣[1] 朱子玲[1] 顾婷婷[1] 金正明[1] 唐晓文[1] 付铮铮[1] 仇惠英[1] 何广胜[1] 苗瞄[1] 马骁[1] 刘跃均[1] 李彩霞[1] 

机构地区：[1]苏州大学附属第一医院江苏省血液研究所,江苏苏州215006

出　　处：《中国实用内科杂志：临床前沿版》2006年第2期288-289,共2页

基　　金：江苏省医学重点人才基金项目(No.RC2002033);苏州市科教兴卫项目(SWQ10)

摘　　要：目的观察在不同类型造血干细胞移植中巨细胞病毒感染状况。方法应用半巢式PCR技术定期检测了2001-03～2003-07苏州大学附属第一医院住院病人55例移植病人外周血标本，比较不同类型移植CMV-DNA阳性率的差别。结果在检测的462份标本中，有285份为阳性，33／55（60％）患者CMV-DNA阳性，非清髓性造血干细胞移植（NST）的阳性检出率为15／17（88．2％），亲缘间外周血造血干细胞移植（PBSCT）的阳性检出率为3／7（42．9％），非亲缘性骨髓移植（UR-BMT）的阳性检出率为7／11（63．6％），亲缘性骨髓移植（R-BMT）的阳性检出率为8／20（40．0％）。NST与PBSCT、R-BMT的巨细胞病毒感染率差别显著（χ^2=5．44，P〈0．05；χ^2=9．14，P〈0．05），NST与UR-BMT（χ^2=2．39，P〉0．05）间的差别无统计学意义。结论巨细胞病毒感染在不同的造血干细胞移植方式可能存在差异。Objective To monitor the incidence of CMV infection in different stem cell transplantation. Methods From Mar, 2001 to Jul, 2003, using nested - PCR, we have detected the CMV - DNA in the 55 patients in the First Affiliated Hospital to Soochow University after different stem cell transplantation and compared the incidence of infection of CMV in different stem cell transplantation. Results In the 462 blood samples, there were 285 blood sample which we detected CMV - DNA. The infection rate in all the patients whom we have studied was 33/55 （ 60. 0% ） ; in the group of nonmyeloablative allogeneic stem cell transplantation, it was 15/17 （88.2%） ; in the group of peripheral stem cell transplantation, it was 3/7 （42. 9% ） ; in the group of unrelated bone marrow transplantation, it was 7/11 （63.6%） ; in the group of related bone marrow transplantation it was 8/20 （40. 0% ） . The infection rate of CMV in the group of nonmye- loablative allogeneic stem cell transplantation was significantly higher than those in related bone marrow and peripheral stem cell transplantation （ χ^2 = 5.44, P 〈 0. 05 ; χ^2 = 9. 14, P 〈 0. 05 ） . The difference of infectious rate of CMV in the group of unrelated bone marrow transplantation and nonmyeloablative allogeneic stem cell transplantation was not statistically significant. （ χ^2 = 2, 39, P 〉 0. 05 ） . Conclusion The incidence of CMV infections may be varied in different stem cell transplantation schemes.

关 键 词：造血干细胞移植 半巢式 PCR 巨细胞病毒 

分 类 号：R5[医药卫生—内科学]