IL-8和NF-κB在慢性阻塞性肺疾病缓解期气道炎症中的作用  

The role of IL-8 and NF-κB in the airway inflammation of COPD in stable stage.

作　　者：吴纪珍[1] 齐咏[1] 马利军[1] 李素云[2] 

机构地区：[1]河南省人民医院呼吸科,河南郑州450003 [2]河南中医学院附属医院呼吸科,河南郑州450007

出　　处：《中国实用内科杂志：临床前沿版》2006年第5期752-754,共3页

基　　金：河南省杰出青年科学基金(0512000700)

摘　　要：目的探讨白细胞介素-8(IL-8)和NF-κB在慢性阻塞性肺疾病(COPD)缓解期气道炎症中的作用。方法选择2003年3月至2005年9月河南省人民医院呼吸科COPD稳定期患者26例,诱导痰检测痰上清液IL-8水平,分离巨噬细胞并以免疫细胞化学法测定NF-κBP65的活性表达,检测外周血超氧化物歧化酶(SOD)、丙二醛(MDA)、IL-8水平,所有患者均测定肺功能(FEV1占预计值%、FEV1/FVC)。结果COPD稳定期诱导痰上清液IL-8水平显著高于正常组(P<0.01);巨噬细胞NF-κBP65胞核阳性表达显著高于正常组,痰IL-8与巨噬细胞NF-κB的激活呈正相关,而与FEV1占预计值%呈负相关(P<0.01);外周血IL-8与对照组比较差异无显著性,但MDA显著高于对照组,SOD低于对照组。结论细胞因子IL-8和NF-κB在COPD缓解期诱导痰中有较高水平,提示IL-8在COPD气道炎症、气道阻塞发生发展过程中可能起着重要作用。注重COPD稳定期的治疗(抗氧化及气道内吸入抗炎药)可能会延缓COPD的发展。Objective To discuss the role of IL - 8 and NF -κB in the airway inflammation of COPD in stable stage. Methods From Mar. 2003 to Sep. 2005, select 26 COPD patients in stable stage, determine the concentration of IL - 8 in the serum of induced sputum, separate the macropolycyte and determine the degree of active expression of NF - κB, the concentration of SOD, MDA and IL-8 in peripheral blood. Lung function （the percent of FEV1/FEV1 prospectived value, FEV1/FVC） of all patients were investigated. Results The concentration of IL - 8 in the serum of induced sputum of COPD patients was higher than the normal group obviously （ P 〈 0. 01 ）. The degree of active expression of NF - κB P65 positive karyon in macropolycyte was much higher than normal group. IL - 8 in sputum had positive correlativity with activity of NF - κB, while negative correlativity with the percentage of FEV1/FEV1 prospectived value （ P 〈 0. 01 ）. The concentration of IL - 8 in peripheral blood had no obvious difference with the control group, while the MDA was much higher and the SOD was lower in COPD patients than control group. Conclusion The level of IL - 8 and NF - κB is higher in induced sputum of COPD patients in stable stage. That hints us IL - 8 possiby plays an important role in occurrence and progress of the airway inflammation and obstruction of COPD. The progress of COPD may be delayed by more attention payed on the treatment in stable COPD antioxidation and inhalation of anti - inflammation.

关 键 词：肺疾病 慢性阻塞性 IL-8 核转录因子 诱导痰 气道炎症 

分 类 号：R5[医药卫生—内科学]