全反式维甲酸联合γ-干扰素对神经母细胞瘤细胞诱导分化及生长抑制作用的研究  

ATRA combined with IFNγ to induce differentiation and growth inhibition of NB cells.

作　　者：杨艳敏[1] 车玉琴[2] 卢岩[3] 佟海霞[3] 李慧[1] 何平[1] 

机构地区：[1]中国医科大学附属第二医院老年科,辽宁沈阳110004 [2]中国医科大学附属第四医院神经内科,辽宁沈阳110032 [3]中国医科大学附属第二医院检验科,辽宁沈阳110004

出　　处：《中国实用内科杂志：临床前沿版》2006年第5期755-757,共3页

基　　金：辽宁省教育厅基金(05L488)

摘　　要：目的体外观察全反式维甲酸(alltransretinoicacid,ATRA)与γ-干扰素(IFNγ)对神经母细胞瘤(neuroblastoma,NB)细胞系SY5Y细胞生长及分化的影响及其可能的分子机制。方法中国医科大学附属第二医院2005年2月至2005年11月用RT-PCR方法检测ATRA、IFNγ及ATRA+IFNγ作用后SY5Y细胞TrKAmRNA表达情况;采用台盼蓝拒染法检测ATRA、IFNγ以及ATRA+IFNγ对SY5Y细胞体外抗增殖作用;用倒置相差显微镜观察细胞形态变化。结果ATRA和IFNγ作用后SY5Y细胞TrKAmRNA表达增高;IFNγ与ATRA对SY5Y细胞均有一定抗增殖作用和诱导细胞分化作用,且二者联合有协同效应。结论IFNγ与ATRA对SY5Y细胞生长抑制及诱导分化有协同作用,该作用与上调SY5Y细胞TrKAmRNA表达水平有关。Objective To study the effect of ATRA and IFNγ on growth and differentiation of SH - SYSY cell line and the possible molecular mechanisms. Methods The expression of TrkA mRNA was detected by RT - PCR. Trypan blue exclusion was used to detect the antiproliferative effect of ATRA, IFNγ and ATRA ＋ IFNγ on SYSY cells. Morphologic changes were observed using phase - contrast microscopy. Results TrkA mRNA was upregulated by ATRA and IFNγ. ATRA and IFNγ could induce morphological differentiation and inhibit growth of SY5Y cells. The combination of ATRA ＋ IFNγ enhanced the induced differentiation and growth inhibition of SYSY cells. Conclusion Synergistic effects of ATRA and IFNγ on growth inhibition and inducing differentiation in SY5Y cells are observed and this may result from upregulating the expression of TrkA mRNA.

关 键 词：神经母细胞瘤 全反式维甲酸 Γ-干扰素 细胞分化 生长抑制 

分 类 号：R5[医药卫生—内科学]