洋金花有效成分的微波提取最佳工艺考察  

Studies on Optimal Process for Active Compounds of Flos Datura by Microwave-assisted Extraction

作　　者：彭拓华[1] 赵子凤[2] 张少俊[1] 黄湘兰[1] 曾凡[1] 

机构地区：[1]广东省药物研究所,广州510440 [2]广东药学院,广州510123

出　　处：《广州中医药大学学报》2006年第3期265-267,271,共4页Journal of Guangzhou University of Traditional Chinese Medicine

基　　金：广东省社会发展攻关基金资助项目(编号:2003B31705);广州市科技攻关重点项目(编号:2005Z2-E5071)

摘　　要：【目的】优选洋金花有效成分的微波提取最佳工艺条件。【方法】采用均匀设计法考察微波功率、微波辐射时间、溶剂用量、浸泡时间、萃取温度等参数对提取洋金花干膏、总生物碱、东莨菪碱效果的影响;采用酸性染料比色法、高效液相色谱法分别测定洋金花总生物碱、东莨菪碱的含量,用质量法测定干浸膏得率。【结果】洋金花微波提取的最佳工艺条件是用药材10倍量的体积分数为75%乙醇浸泡2.0 h,在功率为730 W,温度为65℃的微波条件下连续辐射25 min。【结论】与浸渍法相比,微波提取在提高生产效率和节省溶剂方面占有优势。[ Objective] To optimize the process conditions [ Methods ] Uniform design was used to observe for active compounds of Flos Datura by microwave-assisted the effects of microwave power, microwave radiation time, solvent volume, soaking time and extracting time on the extraction of dry extract, total alkaloids, scopolamine of Flos Datura. The contents of total alkaloids and scopolamine of Flos Datura were determined by HPLC, and the obtaining rate of dry extract was measured by weight method. [Results] The acid stain colorimetry and optimal process conditions for Flos Datura were as follows： soaking the medicinal material for 2 hours with 10 times of 75% alcohol, with continuous radiation for 25 rain at the power of 730 W, at 65℃. [ Conclusion] Microwave-assisted extraction is superior to soaking extraction method in increasing productivity and economizing the solvent.

关 键 词：洋金花/生产和制备 洋金花/化学 总生物碱/分析 东莨菪碱/分析 微波提取 

分 类 号：R284.2[医药卫生—中药学]