丁基锡化合物与人血清白蛋白相互作用的研究  

Study on Interaction of Butyltin Compounds with Human Serum Albumin

作　　者：张朝红[1] 董殿波[1] 臧树良[2] 耿兵[1] 冯冲[1] 陈中林[1] 苏欣[1] 赵广富[2] 

机构地区：[1]辽宁大学环境科学系,沈阳110036 [2]辽宁大学化学系,沈阳110036

出　　处：《化学学报》2006年第9期953-958,共6页Acta Chimica Sinica

基　　金：国家自然科学基金(No.20271024);辽宁省教育厅自然科学基金(No.05L154)资助项目

摘　　要：通过紫外、荧光和CD光谱,研究了船体防污漆中主要防污成分——三丁基锡(TBT)化合物及其降解产物——一丁基锡(MBT)和二丁基锡(DBT)与人血清白蛋白(HSA)相互作用的方式与程度,考察了浓度、酸度、有机溶剂和离子强度等因素对相互作用的影响.结果表明,丁基锡与HSA的相互作用是双重的,既有丁基锡中锡离子与HSA的配位作用,又有丁基锡中丁基基团的疏水作用,导致HSA二级结构破坏,α-螺旋结构减少和构象转变.一般情况下,MBT以配位作用为主,TBT以疏水作用为主,DBT两者兼而有之.The interaction of tributyltin （TBT）, the main components of antifouling paint of ships, and its degradation products of monobutyltin （MBT） and dibutyltin （DBT） with human serum albumin （HSA） was investigated by ultraviolet, fluorescence and circle dichromism spectra. The influences of the concentration, acidity, organic solvent such as ethanol and ionic strength on the interaction of butylrin compounds and HSA molecule were examined to find that the interaction of butyltin compounds and HSA was dual. The butyltin compounds had not only coordinate but also hydrophobic actions with HSA molecule, which led to the destruction of the secondary structure of HSA molecule, decrease of content of a-spiral structure and transformarion of conformation. Generally, MBT is inclined to act with HSA molecule through coordination action, while TBT is inclined to do with HSA molecule through hydrophobic action, and DBT can act with HSA molecule adopting both actions synchronously.

关 键 词：丁基锡化合物 人血清白蛋白(HSA) 相互作用 

分 类 号：R114[医药卫生—卫生毒理学]