克菌丹和灭菌丹的气相和液相色谱分析方法  

Study on the quantitative analysis of captan and folpet by GC and HPLC

作　　者：慕卫[1] 刘峰[1] 孙作洋[1] 刘彦良[1] 潘金菊[1] 

机构地区：[1]山东农业大学植物保护学院,泰安271018

出　　处：《分析试验室》2006年第5期107-109,共3页Chinese Journal of Analysis Laboratory

基　　金：山东农业大学博士后基金

摘　　要：采用气相色谱法和高效液相色谱法两种方法分别对克菌丹和灭菌丹进行定性定量分析. 气相色谱法均以邻苯二甲酸二戊酯为内标物, 色谱柱采用3%OV-101 chromosorb AW DMCS 150～177 μm, 1 m×3 mm玻璃柱, 柱温为195 ℃, 汽化温和检测温均为230 ℃, 克菌丹和灭菌丹的回收率分别为99.2%～100.7%和98.8%～99.9%;方法相对标准偏差分别为0.46%和0.59%. 高效液相色谱法中使用Nova-PaK C18 250 mm×4.6 mm色谱柱, 以V（甲醇）：V（水）＝85：15为流动相, 检测波长为225 nm. 克菌丹和灭菌丹的回收率分别为99.5%～100.6%和98.9%～99.7%;方法相对标准偏差分别为0.68%和0.51%.The quantitative analysis of captan and folpet by GC and HPLC was studied. Gas chromatography was performed on a 3%OV-101 Chromosorb AW DMCS （150 - 177μm） glass column （ 1 m × 3 mm） at a column temperature of 195 ℃ and a detector temperature of 230 ℃ with diamyl phthalate as an internal standard. Recoveries of this method were 99.2 - 100.7% for captan and 98.8 - 99.9% for folpet, and variation coefficient was 0.46% and 0.59%, respectively. HPLC was canied out on a Nova-pak C18 column （250 mm × 4.6 mm） with Vmethanol： Vwater85：15 as mobile phase at a detector wavelength of 225 nm. Recoveries were 99.5 - 100.6% for captan and 98.9- 99.7% for folpet, and variation coefficient was 0.68 % and 0.51%, respectively.

关 键 词：气相色谱 液相色谱 分析 克菌丹 灭菌丹 

分 类 号：O657.7[理学—分析化学]