山奈黄素荧光分光光度测定痕量锗的新方法  

Fluorometric determination of germanium with kaempferol

作　　者：陈雁君[1] 孙平[2] 卢英华[1] 张建萍[1] 全先高[1] 贾少辉[1] 

机构地区：[1]济宁医学院分析实验室,济宁272013 [2]济宁市第一人民医院,济宁272013

出　　处：《分析试验室》2006年第5期88-90,共3页Chinese Journal of Analysis Laboratory

摘　　要：锗与山奈黄素在H3PO4介质中发生配合反应, 形成配合比为2：1的配合物, 此配合物在乙醇溶液中具有很强的荧光强度, λex=438 nm, λem=478 nm. 用山奈黄素荧光分光光度法测定痕量锗, 检出限为0.15 μg/L, 线性范围为1.0～50 μg/L. 高、中、低3种浓度锗的相对标准偏差分别为 0.85%、 2.5%、 2.8%, 加标回收率为95.7%～103.9%.A highly sensitive and fairly selective fluorometric determination method of germanium with kaempferol was developed. Fluorometric reaction of germanium and kaempferol forms a complex in phosphoric acid, and shows intense fluorescence in ethanol solution. The molar ratio of germanium to kaempterol in the complex is 2 ： 1. The complex has an excitation maximum at 438 nm and an emission maximum at 478 nm. The detection limit of germanium was found to be 0.15 μg/L. The linear range was 1.0 - 50 μg/L. The relative standard deviations obtained for the 11 replicate measurements of germanium of high, medium and low concentrations within the linear range were 0.85 %, 2.5 %, 2.8%, respectively. Recoveries of germanium in mushroom were in the range of 95.7% - 103.9%. The effect of several ions was not serious.

关 键 词：锗 山奈黄素 荧光分光光度法 

分 类 号：O657.3[理学—分析化学]