耐甲氧西林金黄色葡萄球菌耐药基因及耐消毒剂基因的研究  

Drug-resistant Gene and Disinfectant-resistant Gene for MRSA

作　　者：胡志东[1] 李金[1] 马睿[1] 徐海茹[1] 田彬[1] 周斌[1] 岳娜[1] 

机构地区：[1]天津医科大学总医院,天津300052

出　　处：《中华医院感染学杂志》2006年第5期481-484,共4页Chinese Journal of Nosocomiology

摘　　要：目的对耐甲氧西林金黄色葡萄球菌(MRSA)进行细菌耐药性分析及耐药基因和耐消毒剂基因的试验研究。方法对临床分离47株MRSA进行菌株鉴定、药敏试验和β-内酰胺类耐药相关基因(mecA),氨基糖苷类耐药相关基因[aac(6′)/aph(2″)、aph(3′)-Ⅲ、ant(4′,4″)],耐消毒剂基因[qac(A/B)]等主要耐药基因和耐消毒剂基因的检测。结果47株MRSA对抗菌药物氨苄西林/舒巴坦、头孢西丁、红霉素、苯唑西林、青霉素均耐药,其他抗菌药物耐药率分别为克林霉素(87.0%)、庆大霉素(91.3%)、利福平(28.3%)、四环素(76.1%)、复方新诺明(65.2%)、左氧氟沙星(71.7%)、呋喃妥因(0.0)、万古霉素(0.0);47株MRSA共检出mecA基因46株,aac(6′)/aph(2″)基因39株,aph(3′)-Ⅲ基因30株,ant(4′,4″)基因6株,qac(A/B)基因19株。结论MRSA为多重耐药菌,对氨基糖苷类耐药和耐消毒剂分别与携带氨基糖苷类耐药基因和耐消毒剂基因有关,临床医生应重视MRSA的诊断与治疗,加强MRSA的医院感染控制。OBJECTIVE To study the drug resistance, drug-resistant genes and disinfectant-resistant genes of MRS A. METHODS The drug resistance and mecA gene of the β-lactamase and aac （6′）/aph（2＂）, aph（3′）-Ⅲ , ant（4′, 4＂） genes of aminoglycoside and qac （A/B） disinfectant-resistant genes were detected in 47 strains of MRSA. RESULTS In all 47 strains of MRSA, 46 MRSA isolates were mecA positive, 39 MRSA isolates were aac （6′）/aph （2＂） positive, 30 MRSA isolates were aph （3′）-Ⅲ positive, 6 MRSA isolates were ant （4′,4＂） positive, and 19 MRSA isolates were qac（A/B） positive. CONCLUSIONS MRSA is multiple-drug resistant. The main resistant mechanisms of MRSA to aminoglycosides and disinfectant are related to the drug-resistant genes of aminoglycoside and disinfectant-resistant genes. Clinic physician must pay attention to the diagnosis and therapy of MRSA, and control the hospital infection.

关 键 词：耐甲氧西林金黄色葡萄球菌 耐药基因 消毒剂 耐消毒剂基因 

分 类 号：R378.11[医药卫生—病原生物学]