GC/MS法测定尿中的8-羟基脱氧鸟苷  

Analysis of 8-hydroxydeoxyguanosine in human urine by gas chromatography/mass spectrometry

作　　者：梅素容[1] 王鹏[1] 吴采樱[2] 周宜开[1] 

机构地区：[1]华中科技大学同济医学院教育部环境与健康重点实验室,湖北武汉430030 [2]武汉大学化学与分子科学学院,湖北武汉430072

出　　处：《华中科技大学学报（自然科学版）》2006年第5期118-120,共3页Journal of Huazhong University of Science and Technology(Natural Science Edition)

基　　金：湖北省自然科学基金资助项目(2003ABA060);中国博士后科学基金资助项目(200435635)

摘　　要：利用气相色谱／质谱（GC／MS）法测定尿中的8-羟基脱氧鸟苷（80HdG）含量．根据MS的定性结果，强峰m／z383对应的碱基＋1（B＋1）为80HdG的特征离子峰．利用选择性离子扫描方式（SIM）进行定量分析，测定结果重复性较好，整个分析流程的系内相对标准偏差为4．23％，系间相对标准偏差为8．25％．该方法的检测限可达0．5nmol／L。线性范围为5～10000nmol／L．分析尿样的相对标准偏差为5．12％，并测定了正常人和癌症病人尿中80HdG的排放水平，癌症病人尿中80HdG的排放水平明显高于正常人．A method of analysis of 8-hydroxydeoxyguanosine （8OHdG） in human urine by gas chromatography/mass spectrometry was developed. On the basis of the qualitative analysis of MS, the most intense ion at m/z 383 corresponding to the base＋ 1 （B＋ 1） was the characteristic ion of 8OHdG. When the selected ion mode （GC/MS-SIM） was chosen as the quantitative method, the detection limit of this method was 0.5 nmol/L, and the linear range was 5410 000 nmol/L. The average interday and interday coefficients of the variation of the method were 4.23 % and 8.25%, respectively. For analysis of urinary 8OHdG, the recovery of spiked urine is 99.37 % and the RSD is 5.12 %. Using the developed method, the excretion levels of urinary 8OHdG of two healthy Chinese persons and six patients from three kinds of cancer were determined. It was found that the excretion levels of urinary 8OHdG in cancer patients were significantly higher than that of urinary 8OHdG in healthy persons.

关 键 词：8-羟基脱氧鸟苷 气相色谱/质谱 选择性离子扫描方式 尿样 癌症 

分 类 号：O657.7[理学—分析化学]