原代猪前体脂肪细胞培养方法的优化  

Optimal Culture Method of the Porcine Preadipocyte

作　　者：李影[1] 杨公社[1] 卢荣华[1] 孙世铎[1] 

机构地区：[1]西北农林科技大学动物脂肪沉积与肌肉发育实验室,杨凌712100

出　　处：《细胞生物学杂志》2005年第6期697-700,共4页Chinese Journal of Cell Biology

基　　金：国家重点基础研究发展规划(973计划)项目资助(No.2004CB117506)~~

摘　　要：以胎猪皮下脂肪组织为材料,比较不同培养方法、消化酶、筛网孔径和离心力对培养猪前体脂肪细胞的影响。结果表明,组织块法与消化法均可培养出猪前体脂肪细胞,但组织块法培养的原代细胞中分化为成熟的细胞较少,消化法获得的细胞均匀一致,形态学染色鉴定大部分均可分化为脂肪细胞;在用消化法培养的过程中,采用Ⅳ型胶原酶消化脂肪组织,200目筛网孔径、800g离心力离心5min可获得大量的、纯度均一的细胞。可以认为,实验成功建立了猪前体脂肪细胞培养的优化体系,在体外重现了猪前体脂肪细胞增殖肥大的全过程。Compared the effect of different culture methods, collagenase, mesh aperture and centrifugal force on the swine preadipocyte in primary culture. The results were as follows： the tissue mass and the digested method were all able to outgrow pig preadipocyte, but the mature adipocyte number was less from the tissue mass than the digested method, and the cells were highly homogeneous, proliferative, and differentiating from the digested method, which were mature adipocytes by oil red o staining identification. During the culture by digested method, the Ⅳ collagenase was used for the first time, the 200 item mesh, 800 g centrifugal force, 5 min could get lots of, homogeneous porcine preadipocyte. In conclusion, we established successfully the optimal system of primary porcine preadipocyte culture, and redisplay the whole process of the proliferative and hypertrophy of porcine preadipocyte.

关 键 词：猪 前体脂肪细胞 培养方法 

分 类 号：Q813.11[生物学—生物工程] S852.2[农业科学—基础兽医学]