薰衣草细胞悬浮培养体系的优化  

The Optimization of Cell Suspensiong Culture Protocol of Lavandula angustifolia, cv. Munstead

作　　者：许耀祖[1] 王晓军[1] 赵民安[1] 韦彦余[1] 赵海清[1] 林侃[1] 

机构地区：[1]中国科学院新疆理化技术研究所

出　　处：《细胞生物学杂志》2005年第6期701-704,共4页Chinese Journal of Cell Biology

基　　金：中国科学院西部行动计划资助项目(No.KGCX2-SW-506)~~

摘　　要：采用正交设计方法对影响薰衣草悬浮细胞生长的因素进行了优化研究,筛选了最有利于薰衣草悬浮细胞生长的培养条件:含有0.025mg/L2,4-D、0.5mg/L6-BA、40g/L蔗糖的B5培养基和120r/min的转速,在此条件下培养15天,悬浮活细胞密度可达到4.4×105个/ml;经过3个月的培养,悬浮细胞的分化率可达到90%以上。通过分析薰衣草细胞对残糖的消耗规律,探讨了蔗糖浓度影响活细胞密度的原因。By the orthogonalty design, the factors influencing suspension cells growth of L.angustifolia were studied, and best culture conditions which were B5 media supplemented with 40 g/L sucrose, 0.025 mg/L 2,4- D, 0.5 mg/L 6-BA and the rotating speed of 120 r/min were sifted. After 15 days of culture, the density of surviving cells could reach 4.4× 10^5 cell/ml, and differentiation rate of suspension cells could surpass 90% after 3 months of suspension culture. The cause of effects of sucrose concentration on the density of surviving cells was discussed through the analysis of the consumption rule of surplus sucrose.

关 键 词：薰衣草 细胞悬浮培养 正交设计 

分 类 号：Q943.1[生物学—植物学]