家蚕胚胎细胞系BmE-SWU1的建立及其生物学特性  

Establishment and Characterization of Embryo Cell Line of Bombyx mori-SWU1

作　　者：潘敏慧[1] 肖仕全[1] 洪锡钧[1] 鲁成[1] 

机构地区：[1]西南大学蚕学与生物技术学院,农业部蚕桑学重点实验室,重庆400716

出　　处：《细胞生物学杂志》2005年第6期708-712,共5页Chinese Journal of Cell Biology

基　　金：国家自然科学基金(No.30471312);国家攻关计划(No.2005BA711A07);国家重点基础研究发展规划项目(973计划)(No.2005CB121000)资助~~

摘　　要：对家蚕反转期胚胎组织进行一年多的原代培养,分离筛选出了高繁殖细胞群体,建立了BmE-SWU1细胞系。该细胞系以梭形和近圆形细胞为主,杂以少量多突起和巨型细胞。细胞系的倍增时间在28℃时为57.6h,染色体呈短杆状或颗粒状,染色体数目异倍化,具有典型鳞翅目昆虫细胞系的染色体特征。BmE-SWU1细胞系对家蚕核型多角体病毒(BmNPV)高度敏感,半数组织培养感染剂量(TCID50)为2.92415×10-7。Embryo cell line of Bombyx mori-SWU 1 （BmE-SWU 1） was established after one year primary culture of differentiation and filtration. The cell line was mainly constructed with two types： shuttle and spheroid. And a few prominency and huge cells were found in cultivation. The cell population doubling time was 57.6 h at 28 ℃. The chromosome of BmE-SWU1 cells showed typical characteristics of lepidoteran insect chromosome of metaphase without apparent centromere, and was short pole-like and pellet-like. The longest chromosome was about 4 μm. The cells were heteroploidy. The infection of BmNPV on the different insect lines showed that BmE- SWU1 was more sensitive to BmNPV. TCID50 was 2.92415× 10^-7.

关 键 词：家蚕 胚胎细胞系 核型 生长曲线 病毒敏感性 

分 类 号：S881[农业科学—特种经济动物饲养]