参麦有效部位对血管内皮细胞增殖和迁移的影响  

Effects of Active Compounds from Shenmai on the Proliferation and Migration of VascularEndothelial Cell

作　　者：陈铌铍[1] 丁志山[1] 方三华[1] 高承贤[1] 徐莉[1] 沃兴德[1] 

机构地区：[1]浙江中医药大学生命科学系,浙江杭州310053

出　　处：《中医药学刊》2006年第5期840-842,共3页Study Journal of Traditional Chinese Medicine

基　　金：浙江省自然科学基金资助项目(399009);浙江省中医管理局科研基金资助项目(2000C11)

摘　　要：目的：探讨参麦中提取的总皂苷、总多糖对血管内皮细胞增殖和迁移的影响.方法：用MTT法检测不同浓度的总皂苷、总多糖及总皂苷＋总多糖对ECV-304、胃癌SGC-7901细胞增殖及SGC-7901条件培养液诱导ECV-304增殖的影响;用琼脂糖刮除法检测它们对血管内皮细胞迁移的影响.结果：当参麦总多糖浓度为0.18～2.44mg/mL时,对ECV-304、胃癌SGC-7901增殖及SGC-7901诱导ECV-304增殖均无影响;当参麦总皂苷浓度为0.05～0.8mg/mL时,对胃癌SGC-7901增殖无影响,而对ECV-304及SGC-7901条件培养液诱导ECV-304增殖有抑制作用,抑制率为13.16%～62.77%.参麦总多糖对ECV-304迁移没有影响,参麦总皂苷对ECV-304迁移有明显抑制作用,抑制率为42%～80%.总皂苷＋总多糖抑制ECV-304增殖和迁移作用与参麦总皂苷相比差异无显著性.结论：参麦中提取的总皂苷对血管内皮细胞和胃癌细胞条件培养液诱导的血管内皮细胞增殖具有抑制作用,并能抑制血管内皮细胞的迁移,是参麦抗血管生成的有效部位.Objective： To observe the effects of total saponins and total polysaccharides extracted from shenmai on the proliferation and migration of vascular endothelial cell. Methods： The effects of total saponins and total polysaccharides from shenmai on the proliferation of ECV -304 cell and gastric carcinoma SGC -7901 as well as ECV -304 cell induced by SGC -7901 cell were measured by MTT colorimetric assay. Their effects on the migration of ECV -304 cell were investigated by agaros assay. Results： When the concentration of total polysaccharides from shenmai was in the range of 0. 18 - 2.88mg/mL, there was no influence on the proliferation of ECV -304 cell and gastric carcinoma SGC -7901 as well as ECV -304 cell induced by SGC -7901 cell when the concentration of total saponins from shenmai was in the range of 0.05 -0.8mg/mL, there was no influence on the proliferation of SGC-7901 cell, but they were able to inhibit the proliferation of ECV - 304 cell and ECV - 304 cell induced by SGC - 7901 cell. The inhibition was 13.16% - 62.77%. There was no influence of total polysaccharides from shenmai on the migration of ECV - 304 cell , but total saponins from shenmai can inhibit the migration of ECV - 304 cell. The inhibition was 42% - 80%. The compounds of total saponins and total asragalans from shenmai can also inhibit the proliferation and migration of vascular endothelial cell, there were no difference between the compounds and total polysaccharides in statistics. Conclusion： Total saponins from shenmai can inhibit the proliferation of vascular endothelial cell and vascular endothelial cell induced by conditional cultured medium of gastric carcinoma cell, they also can inhibit the migration of vascular endothelial cell. Total saponins from shenmai are active compounds.

关 键 词：参麦 有效部位 内皮细胞 血管生成 

分 类 号：R285.5[医药卫生—中药学]