烟叶烘烤中淀粉酶和淀粉磷酸化酶的活性测定  

Activity Determination of Amylase and Starch Phosphorylase in Tobacco Leaves during Flue-curing

作　　者：王怀珠[1] 杨焕文[2] 郭红英[1] 

机构地区：[1]贵州省烟草科学研究所,贵州贵阳550003 [2]云南农业大学烟草学院,云南昆明650201

出　　处：《河南农业科学》2006年第5期32-34,共3页Journal of Henan Agricultural Sciences

基　　金：云南省烟草公司项目(99A13)

摘　　要：为探讨烘烤过程中烟叶淀粉降解酶活性的测定方法,将烘烤过程中的烟叶样品研磨、离心得到酶粗提液,然后采用3,5-二硝基水杨酸比色法测定淀粉酶活性;采用无机磷比色法可测定淀粉磷酸化酶活性。用考马斯亮兰G-250法可测定2种酶蛋白含量。结果表明,2种测定方法测定结果较准确,较客观地反映了烘烤过程中2种酶的实际活性,且简便易行,一般实验室即可完成。The experiment was carried out to establish a method determining the activity of starch degradation enzymes in tobacco leaves during flue-curing. Tobacco samples were abraded and centrifuged to obtain crude extracts of enzymes. Colorimetric method with 3,5 - dinitrogalicylic acid were ,4 applied to determine the content of maltose, then the activity of amylase was figured out. Colorimetric method with inorganic phosphorus was used to determine the activity of starch phosphorylase and Colorimetric method with coomassie brilliant blue G - 250 was for determining the content of these two enzyme proteins. The result showed that the obtained data were relatively accurate and could objectively reflect the activity of enzymes of the tobacco samples during flue-curing.

关 键 词：烤烟 烘烤 淀粉酶 淀粉磷酸化酶 

分 类 号：S572[农业科学—烟草工业]