小麦抗白粉病基因Pm23分子标记的初步鉴定  

Identification of Molecular Marker of Powdery Mildew Resistance Gene Pm23 in Wheat

作　　者：柴春月[1] 刘红彦[1] 伊艳杰[1] 王振军[1] 王瑞[1] 郅玉宝[2] 

机构地区：[1]河南省农业科学院植物保护研究所 [2]河南省农作物新品种重点实验室,河南郑州450002

出　　处：《河南农业科学》2006年第5期47-50,共4页Journal of Henan Agricultural Sciences

基　　金：河南省杰出青年科学基金项目(004012100)

摘　　要：根据植物抗病基因的保守结构,合成7对特异引物,用感病品种Chanceller作对照,对12个含不同抗白粉病基因的小麦品系进行PCR分析,结果只有1对引物(3LR+3LF)在Pm23的载体品系中扩增出稳定的多态性片段,对该片段进行回收、克隆、测序,全长为253bp。经同源性分析发现,该特异性片段与大麦Ty3-gypsy类反转录转座子基因部分序列有87%的同源性,属于反转录转座子类标记。Based on the conserved domains of the resistance genes, 7 pairs of primers were synthesized and used to amplify the genomic DNAs of 12 wheat lines carrying different powdery mildew resistant genes （Pro genes）and one susceptible control Chanceller. The analysis of PCR products showed that only one pair of primers 3LR ＋ 3LF amplified polymorphic fragments in the line carrying Pro23 gene. Sequence similarity analysis indicated that the special amplified fragments with 253 bp in full length was 87% similar to Hordeum vulgate Ty3 - gypsy retrotransposon- like gene, which was belonged to a retrotransposon-like marker.

关 键 词：小麦 白粉病 抗病基因 分子标记 

分 类 号：S435.12[农业科学—农业昆虫与害虫防治]