静脉药瘾者HIV重叠HBV/HCV感染研究  

Study on co-infection of HIV and HBV/HCV in intravenous drug users

作　　者：李建蓉[1] 王晶[2] 田昆仑[2] 王一心[2] 郭利军[2] 张雷[2] 黄汉菊[1] 

机构地区：[1]华中科技大学同济医学院病原生物系,武汉430030 [2]大理学院基础医学部病原生物实验室

出　　处：《中国公共卫生》2006年第5期513-515,共3页Chinese Journal of Public Health

基　　金：国家自然科学基金资助(30160083)

摘　　要：目的探讨人类免疫缺陷病毒(HIV)重叠乙型肝炎病毒(HBV)/丙型肝炎病毒(HCV)感染时,HIV对机体T辅助细胞亚群的影响及HBV/HCV核酸载量的变化。方法以静脉药瘾者(IVDUs)为研究对象,采用ELISA、免疫层析法和PCR法检测病毒感染指标,用ELISA法和间接免疫荧光法检测T细胞亚群。结果IVDUs的CD3、CD4细胞、干扰素(IFN)-γ和白细胞介素(IL)-4产生减少,IVDUs存在HIV感染时CD3、CD4细胞和IFN-γ产生进一步降低,HIV感染与HBV-DNA和HBeAg表达相关。结论IVDUsT细胞亚群功能减弱,HIV促进HBV复制,重叠感染可进一步干扰辅助性T细胞(TH)1/T2细胞平衡,降低机体细胞免疫功能。Objective To understand T lymphocyte subsets and HBV - DNA/HCV - RNA load with co - infection of HIV and HBV/HCV. Methods A total of 410 Intravenous Drug Users （IVDUs） were recruited with informed consent. Peripheral blood mononuclear cells and sera were separated routinely. Viral markers were detected by ELISA and immunochromatographic tests. The load of HBV -DNA/HCV- RNA was identified by fluorescence quantitative PCR. The level of PHA - induced IFN - γ/IL - 4 was determined by ELISA. T lymphocyte subset was determined by fluorescence immunoassay. Results The level of IFN - γ/IL - 4 and the percent of CD3/CD4 cells in IVDUs were lower than that in the healthy controis （P 〈 0.01）. The level of IFN-γ and the percent of CD4 cells were continuously decreased, when the IVDUs was infected with HIV. HBeAg and the load of HBV - DNA were related to HIV - infected. Conclusion Impaired TH response in IVDUs. Virus - infection continuously altered the TH1/TH2 balance. HIV - infection promotes HBV replication.

关 键 词：重叠感染 静脉药瘾者 T细胞亚群 病毒复制 

分 类 号：R183[医药卫生—流行病学]