口服ND弱毒苗对鸡十二指肠中2种神经多肽阳性细胞的影响  

Effect of oral ND attenuated vaccine on somatostatin-positive cells and substance P-positive cells in the duodenum of chickens

作　　者：严婷[1] 练高建[1] 杨倩[1] 

机构地区：[1]南京农业大学农业部动物生理生化重点开放实验室,江苏南京210095

出　　处：《畜牧与兽医》2006年第2期1-3,共3页Animal Husbandry & Veterinary Medicine

基　　金：国家自然科学基金(30070564)

摘　　要：应用新城疫弱毒苗经消化道黏膜2次免疫鸡后,采用免疫组化的方法检测鸡十二指肠中生长抑素(Somatostatin,SS)阳性细胞和P物质(Substance P,SP)阳性细胞的分布及其数量变化。结果表明,动物首免后第3周,新城疫免疫组SS阳性细胞数量极显著增加(P<0.01),而SP阳性细胞数量显著减少(P<0.05);首免后第5周和第7周,新城疫免疫组和对照组中的2种阳性细胞数差异不显著。The chickens were immunized with Newcastle disease attenuated vaccine by digestive route and the Somatostatin （SS） -positive cells and Substance P （SP） -positive cells in the duodenum were detected with immunohistochemical methods. The results showed that the number of SS -positive cells increased significantly （P 〈 0. 01 ） at 3 weeks after the immunization, wherease the number of SP-positive cells decreased significantly （ P 〈 0. 05 ）. At 5 weeks and 7 weeks after the immunization, the number of SS -positive cells and SP-positive cells had no significant differences compared with the control group.

关 键 词：SS阳性细胞 SP阳性细胞 十二指肠 黏膜免疫 

分 类 号：S858.316[农业科学—临床兽医学]