时间分辨荧光分析技术的研究进展及应用  

The development and application of time-resolved fluorescence assay

作　　者：周伟玲[1] 赵启仁[1] 

机构地区：[1]中国医学科学院中国协和医科大学放射医学研究所实验核医学室,天津300192

出　　处：《国际放射医学核医学杂志》2006年第2期103-106,共4页International Journal of Radiation Medicine and Nuclear Medicine

基　　金：天津市自然科学基金资助项目(043610211)

摘　　要：时间分辨荧光分析(TRFA)技术是一种以镧系元素螫合物为标记物,测量其发射荧光的超灵敏的无放射性污染的标记分析技术,在生物分析中得到了广泛的应用。在近些年,研究发展了酶放大时间分辨荧光分析、时间分辨猝灭分析和分子信标等分析系统,其灵敏度更高,满足了不同的需求,使TRFA技术在临床诊断和科学研究中得到了更广泛的应用。Time-resolved fluorescence assay is a new nnnisotopic and uhrasensitive detection method of using lanthanide chelate as detectable label and detect lanthanide chelate fluorescence, having a great prospect. In recent years, a serial of assay systems have been developed quickly and have more sensitivity, such as enzyme-amplified time-resolved fluorescence assay, time-resolved quenching assay and molecular beacon. They fit for more different requirement, and have heen gntten a broader application in clinical diagnosis and science research.

关 键 词：荧光免疫测定 稀土元素 荧光共振能量转移 

分 类 号：R319[医药卫生—基础医学]