献血员感染HBV窗口期血清学标志分析  

WINDOW-PHASE SEROLOGIC MARKERS IN BLOOD DONORS WITH HBV

作　　者：荆永正 谢在海 高忠华 

机构地区：[1]蓬莱市人民医院中心实验室,山东蓬莱265600

出　　处：《齐鲁医学杂志》2006年第3期239-240,共2页Medical Journal of Qilu

基　　金：山东省科学技术发展基金资助项目(012130118)

摘　　要：目的 探讨献血员感染乙型肝炎病毒（HBV）窗口期血清学标志的表现模式.方法 HBV血清学标志利用酶联免疫吸附试验（ELISA）以Alis多功能全自动酶免疫快速分析仪进行检测.采用聚合酶链反应（PCR）检测HBV DNA.为表述方便,将HBV血清学标记检测项目的表面抗原（HBsAg）、表面抗体（anti-HBs）、e抗原（HBeAg）、e抗体（anti-HBe）、核心抗体（anti-HBc）分别标为1～5,并分别以出现阳性项目的序号为该模式的代码.结果 41例献血员感染HBV窗口期血清病毒学标记模式为：“2”1例（2.44%）,“25”1例（2.44%）,“5”4例（9.76%）,于3～6个月后对34例献血员随访血清病毒学标记模式为：“245”15例（44.12%）,“2”9例（26.47%）,“15”3例（8.82%）,“25”2例（5.88%）,“135”1例（2.94%）,“24”1例（2.94%）.结论 献血员感染HBV窗口期血清免疫学标记的模式较为简单,经3～6个月后其血清免疫学标记的模式仍然较为简单,即使对献血员进行HBV五项筛查也解决不了献血员感染HBV窗口期的问题.Objective To explore the pattern of serologic markers of blood donor with HBV in window phase. Methods Serologic markers were detected by ELISA assay using Alis multi-function automate analyzer, and HBV DNA detected by real time PCR. To simplify the illustration, the five kinds of serologic markers of HBsAg, anti-HBs, HBeAg, anti-HBe and anti-HBc were designated as ＂1＂, ＂2＂, ＂3＂, ＂4＂ and ＂5＂, respectively. The code of the positive serologic marker was used to represent the pattern of serologic marker. Results The patterns of serologic marker of 41 HBV DNA positive blood donors in window phase were; ＂2＂ positive, 1 unit; ＂25＂ positive, 1 unit; and ＂5＂ positive, 4 units. Three to six months later, follow up testing of 34 blood donors showed that 15 units were ＂245＂ positive, 9 units were ＂2＂ positive, 3 units were ＂15＂ positive, 2 units were ＂25＂ positive, 1 unit was ＂135＂ positive and 1 unit was ＂24＂ positive. Conclusion HBV NAT is necessary for blood donor screening as serologic markers assay of HBV is unable to detect some potentially infectious units in window phase.

关 键 词：肝炎病毒 乙型 献血员 免疫酶技术 

分 类 号：R512.62[医药卫生—内科学]