类胰岛素样生长因子结合蛋白1-3介导孕激素在人孕早期的作用  

The Role of Progesterone in Human Early Pregnancy is Mediated by Insulin-like Growth Factors Binding Protein_ 1-3

作　　者：靳松[1] 李尚为[1] 龙江[1] 李蕾[1] 谭宗建[1] 

机构地区：[1]四川大学华西第二医院生殖中心,成都610041

出　　处：《四川大学学报（医学版）》2006年第3期399-403,共5页Journal of Sichuan University(Medical Sciences)

基　　金：国家自然科学基金(批准号39970764);四川省计划生育委员会基金(批准号97-4-3)资助

摘　　要：目的探讨孕激素（P）通过其受体（PR）及类胰岛素样生长因子结合蛋白1-3（IGFBP1-3）介导，对胚泡着床的影响。方法选取早孕5～7周行人流术的健康成年妇女蜕膜和绒毛标本共48例，每一孕周分早、中、晚三个时期，每个时期5～6例。以分泌中期（月经周期第21d）宫内膜标本为对照。采用放射免疫法测定血清P水平；采用免疫组化LsAB法和计算机图像分析法检测IGFBP1-3和PR在围着床期子宫内膜、蜕膜和绒毛滋养层细胞中的表达。结果血清P值从分泌中期逐渐上升，在孕5～6周时呈现一峰值，高于分泌中期水平（P〈0．05）。PR在宫内膜腔上皮和蜕膜细胞中表达与血清P存在相关性，但在腺上皮及胚胎滋养层细胞中的表达与血清P无相关性。IGFBP1-3在子宫内膜腺、腔上皮和蜕膜细胞中的表达与血清P具相关性。但胚胎滋养层细胞中的表达与血清P无相关性。结论P对早期妊娠发生与维持具有重要作用，其作用可能是通过胎盘界面PR及IGFBP1-3的介导来完成的。Objective To determine whether the functional role of progesterone in human early pregnancy is mediated by progesterone receptor（PR） and insulin-like growth factor binding protein 1-3（IGFBP1-3）. Methods Sample collection was conducted in accordance to the principle of informed content. Deeidua and villi were obtained from 48 healthy women in 5-7 weeks of pregnancy, and endometrium in mid-secretory phase was obtained as control. The serum levels of progesterone were detected by radioimmunoassay, and the expressions of IGFBP1-3 and progesterone receptor were detected by immunohistochemistry （LsAB）and quantified by a computer image analysis system. Results Serum progesterone increased gradually and reached peak level in 5-6 weeks, which was significantly higher than that during the mid-secretory phase （P〈0. 05）. The expression of progesterone receptor in luminal epithelium and decidual cells had significant correlations with serum progesterone, but its expression in glandular epithelium and trophoblast cells had no correlations with serum progesterone. IGFBP1-3 in luminal , glandular epithelium, and decidual cells had significant correlations with serum progesterone, but their expression in trophoblast cells had no correlations with serum progesterone. Conclusion Progesterone plays an important role in the support and maintenance of early pregnancy, and its effect may be mediated by progesterone receptor and IGFBP1-3 in decidual-trophoblast interface.

关 键 词：孕激素 类胰岛素样生长因子结合蛋白1-3 早孕 

分 类 号：R714[医药卫生—妇产科学]