过氧化物酶增殖物激活受体γ减少血管紧张素Ⅱ诱导的系膜细胞外基质的积聚及其作用机制  

Inhibitive effect and mechanism of PPARγ on the ECM production of mesangial cells induced by angiotensin Ⅱ

作　　者：孙晶[1] 马骥[1] 顾勇[1] 林善锬[1] 

机构地区：[1]复旦大学附属华山医院肾脏科,上海山东省立医院肾脏内科200040

出　　处：《中华肾脏病杂志》2006年第5期297-302,共6页Chinese Journal of Nephrology

基　　金：国家自然科学基金(30200125)上海市科委基金(03JC14084)

摘　　要：目的探讨过氧化物酶增殖物激活受体(PPAR)γ1对血管紧张素Ⅱ(Ang Ⅱ)诱导的系膜细胞外基质积聚的抑制作用及其机制。方法脂质体转染质粒pIRES2-EGFP-PPARγ1／ WT(野生型)于系膜细胞,给予AngⅡ(10-7 mol／L)刺激48 h。采用RT-PCR检测TGF-β1、PAI-1、 c-fos、c-jun mRNA表达水平。采用ELISA法检测细胞上清液中TGF-β1和FN浓度。Western印迹观察胞浆中I-κB、NF-κB及胞核中NF-κB、pERK蛋白表达水平。利用PPARγ／与PPRE结合活性测定PPARγ1在系膜细胞中的活性。同时以PPARγ激动剂吡格列酮(6μmol／L)、不具有目的基因PPARγ1的空白质粒pIRES2-EGFP和表达功能缺陷-突变型(DN)PPARγ1的质粒pIRES2- EGFP-mPPARγ1／DN作为对照。结果 PPARγ1过表达能显著性抑制Ang Ⅱ诱导的系膜细胞 TGF-β1、PAI-1的mRNA高表达(P<0．05),同时下调c-fos和c-jun mRNA高表达(P<0．05)。转染PPARγ1／WT能显著降低AngⅡ 48 h刺激下细胞上清液中FN和TGF-β1浓度(P<0．05)。在AngⅡ作用下系膜细胞AT1表达增加(P<0．05),PPARγ1可显著性减少AT1的高表达(P< 0．05)。AngⅡ诱导的系膜细胞pERK表达明显升高,PPARγ1可显著性减少pERK表达(P<0．05)。转染PPARγ1／WT能上调AngⅡ刺激下系膜细胞胞浆中I-κB低表达,同时下调NF-κB由胞浆向细胞核的转移(P<0．05)。转染PPARγ1／DN并无上述作用。吡咯列酮具有与PPARγ1相同的显著性效应。PPARγ1／WT转染组PPARγ1的活性明显高于其他组(P<0．05)。结论 PPAR-γ1过表达能够抑制AngⅡ刺激下系膜细胞外基质的聚积,降低AT1受体蛋白表达,具有直接抗硬化的非代谢性效应,其机制可能是通过抑制ERK／AP-1及NF-κB等信号分子的传递。Objective To study the inhibitive effect and mechanism of PPARγ1 on the extracellular matrix （ECM） accumulation of mesangial cells induced by Ang Ⅱ .Methods The plasmid of PPARγ1/WT （wild type） was transfected into mesangial cells. After 48 hours of Ang Ⅱ stimulation, the gene expression of TGF-β1, PAI-1, c-los and c-jun was examined by RT-PCR. Protein levels of p-ERK, I-κB and nucleus/cytosol ratio of NF-κB were estimated by Westen-blot. The concentrations of FN and TGF-β1 were estimated by ELISA. The activity of PPARγ1 was examined by specific PPRE binding activity. Plasmid expressing non-functional dominant negative type of mPPARγ1, pIRES2-EGFP-mPPARγ1/DN （DN）, and blank plasmid, pIRES2-EGFP （Blank） were used as controls. Effects of 6 μmol/L PPARγ agonist pioglitazone （Pio） were also studied. Results The expression of TGF-β1 and PAI-1 mRNA in mesangial ceils induced by Ang Ⅱ was inhibited by PPARγ1 （P 〈 0.05）. The over-expression of PPARγ1 down-regulated the expression of c-fos and c-jun significantly （P 〈 0.05）. The expression of AT1 was enhanced by Ang Ⅱ , whereas it was significantly inhibited by the PPARγ1 （P〈0.05）. FN and TGF-β1 production were decreased by PPARγ1/WT transfection. PPARγ1 significantly up-regulated the low-expressed I-κB of cytoplasm and down-regulated the transfer of NF-κB from cytoplasm to nuclear by Ang Ⅱ （P 〈0.05）. PPARγ1/DN did not show the above effects. Pioglitazone had the same effects as PPARγ1 on these parameters in mesangial cells when treated with AngⅡ medium（P 〈 0.05）. The activity of PPAR1γ in the PPARγ1/WT transfected group was significantly higher than that in other groups （P 〈 0.05）. Conclusions PPARγ1 inhibits ECM accumulation induced by Ang Ⅱ through suppressing the expression of AT1, which involves its inhibitory effects on activation of ERK/AP-1 and NF-κB pathways.

关 键 词：过氧化物酶增殖物激活受体γ1 噻唑烷二酮类 血管紧张素Ⅱ 转染 系膜细胞 

分 类 号：R692[医药卫生—泌尿科学]