AFLP标记在玉米种质资源鉴定中的应用  

Identification of Corn Germplasms by AFLP Markers

作　　者：杜金友[1] 靳占忠[2] 徐兴友 耿立英[1] 王天宇[3] 黎裕[3] 

机构地区：[1]河北科技师范学院生命科学系,河北秦皇岛066600 [2]河北科技大学,石家庄050018 [3]中国农业科学院品种资源研究所,北京100081

出　　处：《西北植物学报》2006年第5期927-932,共6页Acta Botanica Boreali-Occidentalia Sinica

基　　金：河北省自然科学基金(303451)

摘　　要：利用AFLP分子标记技术对55份玉米(Z ea m ay s L.)种质材料进行了研究.结果表明,20对AFLP引物检测到清晰可见的带656条,其中多态性带487条.平均每个引物组合可以检测到24.4条多态性带.将55份玉米材料聚为6组,与材料来源基本一致.结果显示AFLP有利于玉米遗传多样性分析.对来源美国先锋的P ioneer系列与瑞得种群和BSSS种群的关系进行了初步探讨,认为P ioneer系列中有很多材料与瑞得种群有亲缘关系.AFLP was adopted to study 55 germplasm materials, 656 bands were detected with 20 pairs of AFLP primers and 487 of them were polymorphic. On average eachprimer combination could be used to detect 24.4 polymorphic bands. The 55 materials were clustered into 6 groups,which were identical to the groups divided by their origins. These results showed that AFLP was conducive to analyzing the genetic diversity of corn. Further analysis of the relation of the Pioneer population of American origin to Reid and BSSS populations indicated that many materials of Pioneer populations had genetic relations to the Reid populations.

关 键 词：玉米 AFLP 遗传多样性 聚类分析 

分 类 号：Q346.5[生物学—遗传学] S513[农业科学—作物学]