BK_(Ca)通道的表达降低细胞蛋白酪氨酸磷酸化水平  

Expression of large conductance calcium-activated potassium channels decreases cellular protein tyrosine phosphorylation

作　　者：李勃兴[1] 陈明[1] 黄潋滟 赵苗[1] 高天明[1] 

机构地区：[1]南方医科大学基础医学院神经生物学教研室,广州510515 [2]公共卫生与热带医学学院

出　　处：《中华神经医学杂志》2006年第5期454-457,467,共5页Chinese Journal of Neuromedicine

基　　金：国家自然科学基金(30330240;30125013)国家基金委与香港研究资助局联合科研基金(30218004)广东省自然科学基金团队项目(10717)教育部长江学者奖励计划资助项目

摘　　要：目的探讨BKCa通道对细胞蛋白酪氨酸磷酸化水平的调节作用。方法观察转染 BKCa通道及共转染BKCa通道与v-Src对HEK293细胞蛋白酪氨酸磷酸化水平的影响。比较BKCa通道激动剂NS1619和阻断剂paxilline对培养海马神经元蛋白酪氨酸磷酸化水平的影响。结果 HEK293细胞表达BKCa通道后细胞蛋白酪氨酸磷酸化水平降低,同时BKCa通道能够降低转染v-Src 后的细胞蛋白酪氨酸磷酸化水平。BKCa通道激动剂NS1619能够降低海马神经元蛋白酪氨酸磷酸化水平,阻断剂paxilline能增加细胞蛋白酪氨酸磷酸化水平。结论 BKCa通道的表达可以降低细胞蛋白酪氨酸磷酸化水平。Objective To study the regulation of cellular protein tyrosine phosphorylation by large conductance calcium-activated potassium （BKCa） channels. Methods The change of protein tyrosine phosphorylation was detected after transfection of BKc, channels or cotransfection of BKc, channels and v-Src in HEK293 cells. The effects of BKCa channel agonist NS1619 and antagonist paxiUine were compared on the protein tyrosine phosphorylation of hippocampal neurons in vitro. Results Protein tyrosine phosphorylation by endogenous and expressed v-Src tyrosine kinase was reduced markedly by the expression of BKc, channels in HEK293 cells. The cellular protein tyrosine phosphorylation of hippocampal neurons was decreased by BKc, channels agonist NS1619, but increased by BKCa channel antagonist paxilline. Conclusion Expression of BKCa channels decreases cellular protein tyrosine phosphorylation.

关 键 词：大电导钙激活钾通道 蛋白酪氨酸磷酸化 V-SRC 

分 类 号：R341[医药卫生—基础医学]