Mash1在室管膜前下区神经干细胞向神经元分化中的作用  

The role of Mashl in the neuronal differentiation of the neural stem cells in SVZa

作　　者：张治元[1] 杨辉[1] 刘仕勇[1] 何家全[1] 邱克军[1] 宋业纯[1] 

机构地区：[1]第三军医大学新桥医院神经外科,重庆400037

出　　处：《中华神经医学杂志》2006年第5期471-474,共4页Chinese Journal of Neuromedicine

基　　金：国家自然科学基金重点课题(30130110)

摘　　要：目的研究Mashl在室管膜前下区(SVZa)神经干细胞向神经元分化中的作用。方法体外分离培养新生0 d昆明小鼠的SVZa神经干细胞,原位杂交检测Mashl在SVZa神经干细胞的表达;构建Mashl与绿色荧光蛋白(GFP)正义、反义融合蛋白表达质粒,转染SVZa神经干细胞,GFP活体荧光标记SVZa神经干细胞;采用细胞计数和流式细胞仪检测在Mashl作用下SVZa神经干细胞分化为神经元的比例。结果体外培养的新生0 d昆明小鼠的SVZa神经干细胞Mashl原位杂交检测阳性;Mashl-GFP+组SVZa神经干细胞分化为神经元的比例明显高于空白对照组;Mashl-GFP-组 SVZa神经干细胞分化为神经元的比例明显低于空白对照组。结论体外培养的新生0 d昆明小鼠的SVZa神经干细胞表达Mashl;Mashl可以促进SVZa神经干细胞向神经元方向分化。Objective To investigate the role of Mashl in the neuronal differentiation of the neural stem cells in SVZa. Methods The neural stem cells derived from SVZa of postnatal Kunming mouse aged 0 day （P0） were separated and cultured in vitro. The mRNA of Mashl in the neural stem cells of SVZa was detected by in situ hybridization. The rate of neuron in the differentiated cells of neural stem cells of SVZa induced by different plasmids of Mashl were detected by cell count and flow cytometry （FCM）. Results The mRNA of Mash l was detected positive in the neural stem cells of SVZa. The rates of neuron in the group of sense Mash 1 were higher than that of blank control group while the rates of neuron in the group of antisense Mashl were lower than those of blank control group. Conclusion Mashl is expressed in the neural stem cells of SVZa and it may promote the neuronal differentiation of the neural stem cells of SVZa.

关 键 词：Mash1 室管膜前下区 神经干细胞 分化 小鼠 

分 类 号：Q421[生物学—神经生物学]