微囊化兔甲状旁腺组织异种移植治疗甲状旁腺功能低下症的实验研究  

Xenotransplantation of Microencapsulated Rabbit Parathyroid Tissue for Treatment of Hypoparathyroidism In Rats

作　　者：高长路[1] 武林枫[1] 刘颖新[1] 翟博[1] 

机构地区：[1]哈尔滨医科大学附属第一临床医学院普外二科,哈尔滨150001

出　　处：《中国普外基础与临床杂志》2006年第3期256-259,共4页Chinese Journal of Bases and Clinics In General Surgery

基　　金：黑龙江省自然科学基金项目(编号:D01-13)~~

摘　　要：目的探讨不同部位微囊化兔甲状旁腺组织异种移植治疗大鼠甲状旁腺功能低下症的效果。方法应用微囊技术制备微囊化(海藻酸钠钡生物微胶囊)兔甲状旁腺组织。16只去甲状旁腺的Wistar大鼠按完全随机法分为肾脂肪囊微囊组及腹腔微囊组,每组8只。分别于移植微囊化兔甲状旁腺组织后5、15、25、35、45、55及65d检测血清钙含量,并于移植后第65d取移植物行透射电镜检查。结果移植后,肾脂肪囊微囊组大鼠血清钙水平恢复到正常范围直至观察结束时,透射电镜检查显示移植物存活良好;腹腔微囊组大鼠血清钙水平恢复到正常范围,但第65d时血清钙水平下降至<1.6mmol/L,透射电镜检查显示移植物中心坏死,仅边缘有少数存活细胞。结论微囊化兔甲状旁腺组织异种移植在不用免疫抑制剂情况下,可以在大鼠体内存活,且有功能。不同移植部位移植后随着时间的延长存在差异,肾脂肪囊在微囊化异种组织移植中是首选的移植部位。Objective To investigate the effect of xenotransplantation of microencapsulated rabbit parathyroid tissue in different sites in rats for the treatment of hypoparathyroidism. Methods The parathyroid glands from Wistar rats were removed to make them aparathyroid. Ultimately, sixteen rats were included because their serum calcium values were continuously below 1. 6 mmol/L. We also encapsulated the cultured rabbit parathyroid tissue with alginate-BaCl2 microcapsule. According to the transplantation sites, rats were randomly divided into two groups： renal adipose microcapsule group and peritoneal microcapsule group, eight in each group. Encapsulated rabbit parathyroid tissues were then transplanted accordingly to different microcapsule groups. The calcium serum contents were examined on 5,15,25,35,45,55 and 65 d respectively after transplantation and the grafts were observed through electron microscope on the 65 d in particular. Results The calcium contents after transplantation in renal adipose microcapsule group restored to normal and the observation outcomes of grafts showed that they survived well. The calcium contents of posttransplantation in peritoneal group also restored to normal with an exception that it dropped to a level lower than 1.6 mmol/L on the 65 d. Electron microscope also showed that there were necrotic tissues in the center and only a few cells survived on the edge of the grafts. Within peritoneal microcapsule group, the values were significantly lower than others taken at different phases. Conclusion Microencapsulated rabbit parathyroid tissue that was xenotransplanted into rats can survive and function without administration of immunodepressant. There are significant differences of calcium contents at varying phases between two transplantation sites, which demonstrate that renal adipose may be an optimal site for microcapsule xenotransplantation.

关 键 词：甲状旁腺组织 异种移植 移植部位 甲状旁腺功能低下症 

分 类 号：R653[医药卫生—外科学]