结直肠癌组织Tob mRNA表达的研究  

Expression of Tob mRNA in Human Colorectal Cancer

作　　者：吴殿超[1] 周总光[1] 郑雪莲[1] 王俊峰[1] 

机构地区：[1]四川大学华西医院普外科,消化外科实验室成都610041

出　　处：《中国普外基础与临床杂志》2006年第3期302-304,共3页Chinese Journal of Bases and Clinics In General Surgery

基　　金：国家杰出青年基金(编号:39925032)~~

摘　　要：目的研究TobmRNA在结直肠癌组织及其相应的距肿瘤10cm以远并经病理学证实的正常组织中的表达,探讨其在结直肠癌发生、发展中的作用。方法用荧光定量RT PCR方法检测31例结直肠癌标本及其相应的正常组织中TobmRNA的表达。结果31例癌组织中TobmRNA表达高于正常组织(t=3.97,P=0.000);TobmRNA表达随着Dukes分期有增高趋势,差异有统计学意义(F=3.366,P=0.033)。结论在结直肠癌的发病机理中,TobmRNA可能为致癌基因。Objective To investigate the expression of Tob mRNA in human colorectal cancer tissues, and their corresponding para-cancerous normal tissues which was 10 cm above the tumor and pathologically proved and to explore the role of Tob mRNA in the pathogenesis of colorectal cancer. Methods Quantitative real time RT-PCR was used to detect the expression of Tob mRNA in 31 colorectal cancers. Results Compared with para-cancerous tissue, the expression of Tob mRNA in colorectal cancer tissues was significantly increased. Moreover, the expression levels of Tob in Dukes A, B, C, D were 1. 146±0. 067, 1. 120±0. 073, 1. 052±0. 020 and 1. 047±0. 010 respectively. Analyzed by one-way ANOVA, there were significant differences in expression of Tob in different Dukes stage. Conclusion The up-regulation expression of Tob mRNA may be closely associated with tumorigenesis of colorectal carcinoma.

关 键 词：结直肠癌 Tob MRNA表达 

分 类 号：R735.3[医药卫生—肿瘤]