钙离子载体A23187诱导人外周血单个核细胞生成树突状细胞  

Effect of Calcium Ionophore A23187 on Dendritic Cells Derived From Peripheral Blood Mononuclear Cells

作　　者：孟娟[1] 左学兰[1] 刘小红[1] 杨华强[1] 张克俭[1] 谭细友[1] 陈飞[1] 

机构地区：[1]武汉大学中南医院血液科,武汉430071

出　　处：《武汉大学学报（医学版）》2006年第3期311-314,共4页Medical Journal of Wuhan University

摘　　要：目的:研究钙离子载体(CI)A23187诱导健康人外周血单个核细胞(PBMNCs)生成树突状细胞(DCs),探索DCs扩增的新方法。方法:分离健康人PBMNCs,分别加入GM-CSF+IL-4,A23187。体外培养72 h后,于光镜、电镜下观察细胞的形态,流式细胞仪检测细胞的表面标志,MTT比色法检测其对同种异体T细胞的刺激增殖作用,ELISA法检测IL-12、IFN-γ的水平。结果:健康人PBMNCs在A23187的条件下培养72 h后,就可以看到典型的DCs形态,CD40、CD86、CD83分子的表达均明显增高,但CD1α分子的表达增高不明显。具有明显刺激同种异体T细胞增殖的能力。IL-12、IFN-γ的水平比其他组明显增高。结论:健康人PBMNCs在钙离子载体A23187作用下能更有效地诱导生成成熟DCs。Objective： To explore the effect of calcium ionophore （CI）A23187 on dendritic cells （DCs） from healthy human peripheral blood monocytes（PBMNCs）. Methods： PBMNCs from healthy donors were treated with A23187 and rhGM-CSF plus IL-4. After culturation of 72 hours, changes of cellular morphology were observed under light and electron microscopy. Surface makers on DCs were analyzed by flow cytometry. MTT colorimetry was used to detect proliferation of allogenetic T cells. Plasma concentrations of IL-12 and IFN-γ were measured by ELISA. Results： PBMNCs treated with A23187 for 72 hours could observe DCs with typical morphology. There was a simultaneously increase in CD40, CD83 and CD86, but a decrease in CD1α. In addition, it could stimulate evidently proliferation of allogeneic T cells. The levels of IL-12 and IFN-γ were increased compared with other groups （P〈0.05）. Conclusion： A23187 can effectively enhance markedly transformation of peripheral blood monocytes into DCs.

关 键 词：钙离子载体 Γ-干扰素 树突状细胞 外周血单个核细胞 

分 类 号：R392.12[医药卫生—免疫学]