利用分子标记技术选择柚类核心种质资源  

Screening the core collection of pomelo germplasm based on molecular marker

作　　者：刘勇[1,2] 孙中海[1] 刘德春[3] 吴波[3] 周群[4] 

机构地区：[1]华中农业大学柑桔研究所 [2]江西农业大学农学院,南昌330045 [3]江西农业大学农学院 [4]江西省农业厅外资利用办公室

出　　处：《果树学报》2006年第3期339-345,共7页Journal of Fruit Science

基　　金：国家自然科学基金资助项目(30070528);教育部优秀青年教师基金资助项目。

摘　　要：根据678个SSR和AFLP分子标记聚类结果,对110份柚类资源采用逐级压缩法选择核心种质,比较了样本数不同的8个核心样本群的多态性位点数、多态性位点百分率、观测等位基因数、有效等位基因数、Nei’s遗传多样性指数和Shannon’s信息指数等参数,最终选择了25个样本组成的样本群作为核心种质。用简明统计软件(CS10.1)对初始种质和核心种质群体的观测等位基因数、有效等位基因数、Nei’s遗传多样性指数和Shannon’s信息指数分别作t测验,可看出核心种质保留了初始种质22.73%的样品,多态性位点和多态性位点百分率保留率均达到了94.87%,观测等位基因数、有效等位基因数、Nei’s遗传多样性指数、Shannon’s信息指数的保留率分别为97.80%、99.86%、100.72%、101.16%,可见核心种质能很好的代表初始种质。According to the result of 678 SSR and AFLP markers, stepwise reduce method was used to screening the core collection of 110 pomelo germplasm. After compared the parameters of 8 sample groups such as the number of polymorphic loci, percentage of polymorphic loci, and observed the number of alleles, effective number of alleles, Nei＇ s gene diversity and Shannon＇s information index, 25 samples were chosen as core collection. T-test was performed by using CS10.1 software between initial collection and core collection, the results showed that the core collection hold 22.73% samples of initial col- lection, the reserved rate of number of polymorphic loci and percentage of polymorphic loci reached 94.87%, the reserved rate of observed number of alleles, effective number of alleles, Nei＇ s gene diversity and Shannon＇ s information index were reached 97.80%, 99.86%, 100.72%, 101,16% respectively. Those results demonstrated that the core collection could stand for initial collection excellently.

关 键 词：柚类 种质资源 遗传多样性 SSR AFLP 核心种质 

分 类 号：S666.3[农业科学—果树学]