无子西瓜郑抗4号的体细胞胚诱导及植株再生(英文)  

Somatic embryogenesis and plantlet regeneration in Citrullus lanatus cv. Zhengkang No. 4

作　　者：牛姗姗[1] 宋尚伟[1] 闫锋[1] 苗红霞[1] 

机构地区：[1]河南农业大学林学园艺学院,郑州450002

出　　处：《果树学报》2006年第3期406-410,共5页Journal of Fruit Science

基　　金：河南省高校青年骨干教师资助计划项目;河南省沙薄地综合治理项目(20040526)。

摘　　要：以无子西瓜(CitrulluslanatusMansfeld)郑抗4号无菌苗子叶为外植体,进行了体细胞胚发生及植株再生的研究,结果表明,无菌苗应先进行3d暗培养,然后采取光照16h/d和黑暗8h/d培养3d;将子叶的叶面朝下放置于培养基上的愈伤组织诱导率高于叶面朝上的培养方式;子叶诱导胚性愈伤组织的最适培养基配方为MS+6-BA2.0mg/L+KT1.0mg/L+GA31.0mg/L;诱导需要在黑暗条件下启动,进行7d暗培养,而生长分化于光照16h/d和黑暗8h/d条件下培养7d;继代3次得到的胚性愈伤组织最多;最适生根培养基配方为1/2MS+IBA0.3mg/L。体细胞胚胎诱导率最高为25%。胚性愈伤组织经进一步培养发育为成熟的体细胞胚胎,生根成为完整植株。Plantlet regeneration from cotyledon of Citrullus lanatus cv. Zhangkang No. 4 was studied. The results showed that aseptic seedlings should be cultured in dark for 3 d, then exposed to a photoperiod of 16/8 （lignt/dark） h/d for 3 d. The callus induction rate was higher in cotyledons placed facing downwards to the medium than in those facing upwards. The highest induction rate occurred in MS medium containing 6-BA （2.0 mg/L）, kinetin （1.0 mg/L）and GA3 （1.0 mg/L）. The induction initiated in dark and took 7 days, while callus growth and differentiation proceeding for 7 days under 16/8 h light-dark cycles. A highest rate of embryogenic callus was obtained after 3 successive subcultures. The optimum rooting medium was 1/2 MS＋IBA （0.3 mg/L）. The highest induction rate of somatic embryo was 25%. Embryogenic calli developed into mature somatic embryos and plantlets.

关 键 词：无子西瓜 体细胞胚发生 植株再生 

分 类 号：S651[农业科学—果树学]