中林美荷杨不定芽分化的因素分析与植株再生  

Analysis adventitious shoot polarization and plantlet regeneration of Poplar-Zhonglinmeihe

作　　者：曾幼玲[1] 易丽娟[1] 张富春[1] 

机构地区：[1]新疆大学生命科学与技术学院新疆生物资源基因工程重点实验室,乌鲁木齐830046

出　　处：《植物研究》2006年第3期329-332,共4页Bulletin of Botanical Research

基　　金：国家科技攻关西部科技行动项目(No.2001BA901A32)

摘　　要：对中林美荷杨进行组织培养,采用不同外植体为组织培养材料,以6-BA和NAA或IBA不同激素组合,比较1/2MS与MS不同盐浓度培养基诱导不定芽产生的情况。结果表明叶柄不定芽诱导优于茎段和叶片,茎段形成层不定芽诱导效果也较好。叶柄诱导不定芽的最佳培养基及激素组合是:1/2MS+6-BA0.5 mg.L-1+NAA0.05 mg.L-1,较MS基本培养基不定芽生长正常,芽诱导率高(76%),增殖倍数达4.7个/叶柄。通过不定芽的继代培养及壮苗、生根,形成完整植株,炼苗移栽成活率高。Inducing adventitious shoots were produced from different explants of Populus sp. by tissue culture on 1/2MS or MS medium with different hormone arrays which were 6-BA plus NAA or IBA. The results showed that the adventitious shoot inducing rate of leafstalk was higher than leaf and stem, the adventitious shoots polarization of stem cambium was also good. The optimal medium of leafstalk was 1/2MS ＋ 6-BA0.5 mg · L^-1 ＋ NAA0.05 mg · L^-1 titious shoot rate was 76% ,shoot number was 4.7 , adventitious shoots grew very well,inducing advenper leafstalk. Adventitious shoots could become whole plants by adventitious shoots multiplication,strength and radication.

关 键 词：中林关荷杨 外植体 激素 盐浓度 不定芽分化 

分 类 号：S888.31[农业科学—特种经济动物饲养] S792.11[农业科学—畜牧兽医]